Diagnostic performance and application of a real-time PCR assay for the detection of Salmonella in fecal samples collected from hospitalized horses with or without signs of gastrointestinal tract disease

The Veterinary Journal
A B EkiriJ A Hernandez

Abstract

The main objective of this study was to assess the diagnostic performance of a real-time polymerase chain reaction (PCR) assay for the detection of Salmonella in fecal samples collected from hospitalized horses with or without signs of gastrointestinal (GI) tract disease. The PCR assay used primers and a probe that targeted the invA gene of Salmonella. Assuming a sensitivity of 100% and a specificity of 96.6%, and a disease prevalence of 2%, 5%, and 10-15% in study horses, the PCR assay had a high (100%) negative predictive value, and a positive predictive value that ranged from 37% in horses without signs of GI disease that tested Salmonella culture-negative, to 60% in horses with signs of GI disease that tested Salmonella culture-negative, to 76-83% in horses with signs of GI disease that tested Salmonella culture-positive. This study provides evidence that the real-time PCR that targets the Salmonella invA gene can be used as a screening test for the detection of Salmonella in feces of hospitalized horses with signs of GI disease. Horses that test PCR-positive can be tested in series using bacteriologic culture to reduce false positive results or to provide additional data (e.g., antibiogram and serotyping data) that can be ...Continue Reading

References

Apr 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·N D CohenB M Hargis
Jul 1, 1993·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·N D CohenB M Hargis
Mar 30, 2002·Journal of Clinical Microbiology·Claudia Gentry-WeeksMichael Jessen
Sep 13, 2002·American Journal of Veterinary Research·P Brett KurowskiClaudia R Gentry-Weeks
Aug 25, 2004·Journal of the American Veterinary Medical Association·Nicolas S ErnstBen J Miller
Apr 14, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Michael P WardChing Ching Wu
Apr 6, 2006·Journal of Veterinary Internal Medicine·Rebecca C WindsorHilde E V De Cock
Jan 6, 2009·Journal of the American Veterinary Medical Association·Abel B EkiriJorge A Hernandez

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Citations

May 12, 2004·Journal of Feline Medicine and Surgery·S F FosterR Malik
Sep 2, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Laura B GoodmanBelinda S Thompson
Aug 29, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Alysia M ParkerJohn K House
Aug 1, 2020·Frontiers in Microbiology·Carolina VillamilJohn Emerson Leguizamon

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