Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries

PLoS Neglected Tropical Diseases
Piet CoolsBruno Levecke

Abstract

Because the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for each phase of a deworming program the minimal criteria that a potential diagnostic method needs to meet, the so-called target product profiles (TPPs). We compared the diagnostic performance of a single Kato-Katz (reference method) with that of other microscopy-based methods (duplicate Kato-Katz, Mini-FLOTAC and FECPAKG2) and one DNA-based method (qPCR) for the detection and quantification of STH infections in three drug efficacy trials in Ethiopia, Lao PDR, and Tanzania. Furthermore, we evaluated a selection of minimal diagnostic criteria of the TPPs. All diagnostic methods showed a clinical sensitivity of ≥90% for all STH infections of moderate-to-heavy intensities. For infections of very low intensity, only qPCR resulted in a sensitivity that was superior to a single Kato-Katz for all STHs. Compared to the reference method, both Mini-FLOTAC and FECPAKG2 resulted in significantly lower fecal egg counts for some STHs, leading to a substantial und...Continue Reading

References

Jan 23, 2003·Annals of Tropical Medicine and Parasitology·M AlbonicoQ Bickle
Apr 15, 2004·Parasitology International·L SavioliA Montresor
Mar 11, 2010·Methods : a Companion to Methods in Enzymology·Jenny SchlesingerSilke R Sperling
Apr 18, 2013·Asian Pacific Journal of Tropical Biomedicine·Jia-Xu WangLi-Wang Cui
May 16, 2013·PLoS Neglected Tropical Diseases·Zeno BisoffiMarco Albonico
Apr 4, 2014·Clinical Microbiology Reviews·Jaco J Verweij, C Rune Stensvold
Apr 5, 2014·Lancet·Daniel G ColleyCharles H King
Apr 23, 2014·Tropical Medicine & International Health : TM & IH·Olga StaudacherFrank P Mockenhaupt
Jun 16, 2014·Parasites & Vectors·Beatrice BardaMarco Albonico
Nov 6, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·Daniel DanaBruno Levecke
Mar 25, 2017·Transactions of the Royal Society of Tropical Medicine and Hygiene·Chiara MaroccoAntonio Montresor
Apr 21, 2017·PLoS Neglected Tropical Diseases·Hélène CarabinPierre Dorny
Apr 21, 2017·PLoS Neglected Tropical Diseases·Peter Mark JourdanJudd L Walson
Sep 9, 2017·Lancet·Peter Mark JourdanDavid G Addiss
Jan 19, 2018·Transactions of the Royal Society of Tropical Medicine and Hygiene·A MontresorC Fitzpatrick
Mar 2, 2018·PLoS Neglected Tropical Diseases·Mark D LimUNKNOWN Annecy STH diagnostic experts group

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Citations

Apr 15, 2020·The Cochrane Database of Systematic Reviews·Lucieni O ConternoRicardo Augusto Monteiro de Barros Almeida
Dec 13, 2019·The Journal of Infectious Diseases·Luc E CoffengSake J de Vlas
May 30, 2020·Nature Reviews. Disease Primers·Kathryn J ElsePhilip J Cooper
Jul 29, 2020·Animals : an Open Access Journal From MDPI·Fiona TysonRussell M Morphew
Nov 17, 2020·Frontiers in Veterinary Science·Maria Paola MaurelliLaura Rinaldi
Nov 19, 2020·Journal of Parasitology Research·Daniel Kevin WerungaJackson Cheruiyot Korir
Apr 28, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Carolin VegvariRoy M Anderson
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Sangeeta DekaDeepjyoti Kalita
Jul 13, 2021·Parasitology·M P MaurelliL Rinaldi
Aug 12, 2021·Research and Reports in Tropical Medicine·Sumeeta KhuranaAbhishek Mewara
Oct 28, 2021·Tropical Medicine & International Health : TM & IH·Saravanakumar KaliappanSitara Ajjampur

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Methods Mentioned

BETA
PCR

Clinical Trials Mentioned

NCT03465488

Software Mentioned

Mini
FECPAK
FLOTAC
Helminth Egg Automated Detector , ( HEAD )
HEAD
RotorGene

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