Diagnostic performance of different fecal Lawsonia intracellularis-specific polymerase chain reaction assays as diagnostic tests for proliferative enteropathy in pigs: a review

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Ken Steen PedersenJens Peter Nielsen

Abstract

Traditionally, diagnosis of Lawsonia intracellularis-associated proliferative enteropathy (PE) has depended on necropsy and histology. Since the establishment of the etiologic role of L. intracellularis, a number of specific polymerase chain reaction (PCR) assays have been developed for the detection of DNA in feces. The present article is a systematic review of peer-reviewed publications on the application of L. intracellularis-specific fecal PCR as an antemortem diagnostic test for histologic lesions of PE in pigs. Based on this information, a range of diagnostic sensitivities (36-100%) and specificities (50-100%) of the published tests was calculated. Validity and confidence limits of the estimates varied considerably. The positive and negative predictive values of 6 different PCR assays were calculated for PE prevalence of 15%, 30%, 45%, 60%, 75%, and 90%, using a histologic case definition of PE and based on the reported test sensitivities and specificities. The simulated predictive values suggested that applying the fecal PCR assay as a diagnostic test is more likely to overestimate than underestimate the number of pigs having histologic lesions of PE under field conditions.

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Citations

Jun 24, 2011·Clinical and Vaccine Immunology : CVI·Eleanor WatsonDavid G E Smith
Nov 10, 2012·Journal of Animal Science and Biotechnology·Shuhong ZhaoHongbo Chen
Jan 1, 2011·Polish Journal of Veterinary Sciences·A SzczotkaZ Pejsak
Jul 13, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ken Steen PedersenJens Peter Nielsen
Mar 29, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ken Steen PedersenØystein Angen
Jul 6, 2018·Journal of Veterinary Pharmacology and Therapeutics·Denisa Soledad Pérez GaudioAlejandro Luis Soraci
Jan 1, 2020·Porcine Health Management·Mirjam ArnoldHeiko Nathues
Mar 20, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Marta CampilloTanja Opriessnig

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