Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing

Veterinary Microbiology
J M A BrinkhofC van Maanen

Abstract

The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks. The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of <3% within-herd prevalence using 1/10 pre-diluted samples and even <1% within-herd prevalence when samples were tested undiluted. In a panel of 249 bulk milk samples, all samples from SRLV free flocks (n=138) tested negative in the ELISA, while 50% of the samples from flocks with an unknown SRLV-status (n=111) were positive. For a subset of 59 bulk milk samples, agreement between ELISA results and leader-gag PCR results was almost 100%. These results demonstrate the potential of bu...Continue Reading

References

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Citations

Oct 17, 2014·Indian Journal of Microbiology·R AsadpourR Jafari
Apr 25, 2013·Viruses·Hugo RamírezHumberto A Martínez
Feb 5, 2013·Veterinary Immunology and Immunopathology·X de AndrésB Amorena
Sep 16, 2015·Veterinary Microbiology·E MinguijónR A Juste
Feb 27, 2013·Acta Veterinaria Hungarica·Urška KuharJože Grom
Feb 7, 2013·The Veterinary Journal·Bernardo Martínez-NavalónEduardo Berriatua
Jul 25, 2013·Viruses·Juliano Cezar Minardi da CruzYahia Chebloune
Jun 11, 2011·Tropical Animal Health and Production·Mohamed Hassan TageldinSeif S Al-Habsi
Apr 17, 2019·Journal of Veterinary Research·Takfarinas IdresYahia Chebloune

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