Diagnostic performance of isothermal strand displacement amplification of Mycobacterium tuberculosis IS 6110 in tissue samples

International Journal of Mycobacteriology
Amani Alnimr, Areej Alnemer

Abstract

Visualized histopathological findings in tissue samples are not specific for tuberculosis while mycobacterial cultures from such specimens have low yields and long turn around times. A rapid, sensitive method is therefore needed for detection of Mycobacterium tuberculosis in paucibacillary tissue samples. In this paper, a total of 158 tissue specimens, including 42 culture-positives, were tested for the presence of Mycobacterium tuberculosis by strand displacement amplification of DNA targeting the region of the insertion element IS 6110 and detected by a chemiluminescence based commercial platform (BDProbeTec™ ET System). The amplification results were correlated to histopathology, microscopy and microbiological culture. The strand displacement amplification based assay showed low overall sensitivity (31.5%) but high specificity (97.5%) which varied across various tissue types. Only 35.7% of culture-positive biopsies were positive by the molecular assay. Some discrepancy were attributed to suboptimal performance of the traditional methods. The assay is useful to rule in the disease in common tissue specimens (lung, pleura and lymph node); but less so in other tissue types. The poor sensitivity in tissue specimens necessitates ...Continue Reading

References

Apr 11, 1992·Nucleic Acids Research·G T WalkerD P Malinowski
Dec 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·C RichterJ Oosting
Dec 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S DasP R Narayanan
Oct 1, 1995·Cytopathology : Official Journal of the British Society for Clinical Cytology·T KüpperP Pfitzer
Oct 14, 1998·American Journal of Respiratory and Critical Care Medicine·N V SalianJ H Bates
Aug 27, 1999·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·J G BradyD M Parham
Jun 13, 2000·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·J Y LiC S Ng
Oct 3, 2002·American Journal of Respiratory and Critical Care Medicine·Hajime FukunagaTokuhiro Ishihara
Jun 24, 2003·The International Journal of Biochemistry & Cell Biology·Jim F HuggettAlimuddin Zumla
Jul 5, 2003·Journal of Clinical Microbiology·Olga L SarmientoWilliam C Miller
Dec 18, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Mariana Cristea FernstromBjorn Petrini
Jan 30, 2004·Cytopathology : Official Journal of the British Society for Clinical Cytology·A S AljafariA M Elhassan
Nov 23, 2005·Microbes and Infection·Rafael SaavedraLuz M López-Marín
Aug 11, 2006·The European Respiratory Journal·F A DrobniewskiUNKNOWN WHO European Laboratory Strengthening Task Force
Aug 31, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Fernando OsoresCiro Maguiña
May 18, 2007·Journal of Clinical Microbiology·Sahal A M Al-HajojNalin Rastogi
Jan 16, 2009·MMWR. Morbidity and Mortality Weekly Report·UNKNOWN Centers for Disease Control and Prevention (CDC)

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