Abstract
There are growing concerns regarding the spread of carbapenemase-producing organisms (CPOs) among patients in long-term care facilities (LTCFs) and hospitals in South Korea. We have established a screening protocol for the detection of CPOs in high-risk patients upon admission to intensive care units (ICUs). The diagnostic performance of the Xpert Carba-R assay was compared to that of rectal culture for CPO detection in high-risk patients upon ICU admission. A total of 408 consecutive rectal swabs were obtained from December 2016 to December 2017. CPO screening was performed using the Xpert Carba-R assay (Cepheid, Sunnyvale, CA, USA). When a carbapenemase gene was detected, additional rectal swabs were incubated overnight and inoculated on chromID CARBA medium (bioMérieux, Marcy l'Etoile, France). Bacterial carbapenemase genes, including blaKPC, blaNDM, blaVIM, blaIMP-1, and blaOXA-48, were confirmed by conventional PCR. The diagnostic performance of the Carba-R assay was ascertained based on the culture results. The prevalence of CPO carriage was 7.4% according to the Carba-R assay and 3.7% according to rectal culture. The median Ct values of IMP-1 and KPC were significantly different (35.2 vs. 26.6, P = 0.0143). The overall s...Continue Reading
References
Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Laurent PoirelPatrice Nordmann
Feb 14, 2012·International Journal of Antimicrobial Agents·Jung Sik YooYeong Seon Lee
Aug 16, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael Y LinUNKNOWN Centers for Disease Control and Prevention Epicenters Program
Sep 6, 2013·Journal of Clinical Microbiology·Fred C TenoverDavid H Persing
Oct 5, 2013·Diagnostic Microbiology and Infectious Disease·Delphine GirlichPatrice Nordmann
Mar 3, 2015·Annals of Laboratory Medicine·Wonkeun SongIl Kwon Bae
Sep 5, 2015·Journal of Clinical Microbiology·Anna F LauKaren M Frank
Dec 18, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Barbara AlbigerUNKNOWN European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) working group
Dec 29, 2015·Annals of Laboratory Medicine·Do-Kyun KimKyungwon Lee
Feb 26, 2016·Journal of Clinical Microbiology·Thomas J GniadekPatricia J Simner
Apr 8, 2016·Journal of Clinical Microbiology·Patricia J SimnerAaron M Milstone
Apr 29, 2016·Journal of Clinical Microbiology·M TatoR Cantón
Jul 28, 2016·Scientific Reports·Philipp WarnkeAndreas Podbielski
Aug 3, 2016·Antimicrobial Agents and Chemotherapy·Jun HongFei Ke
Nov 22, 2016·The Lancet Infectious Diseases·Hajo GrundmannUNKNOWN European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group
Mar 31, 2017·Journal of Clinical Microbiology·Shelley A MillerRomney M Humphries
Apr 5, 2017·The Journal of Infectious Diseases·Latania K Logan, Robert A Weinstein
Apr 16, 2017·International Journal of Antimicrobial Agents·Yannick Hoyos-MallecotThierry Naas
May 19, 2017·Journal of Clinical Microbiology·Nicholas M MooreUNKNOWN Carba-R Study Team
Feb 27, 2018·Future Microbiology·Eun-Jeong YoonSeok Hoon Jeong
Mar 7, 2018·The Korean Journal of Internal Medicine·Young Ah Kim, Yoon Soo Park
Jun 1, 2018·Journal of Clinical Microbiology·Maria M TraczewskiUNKNOWN Carba-R Study Team
Citations
Jan 3, 2021·Diagnostic Microbiology and Infectious Disease·Eric FarfourUNKNOWN GMC study group
Mar 26, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Jaffar A Al-TawfiqAli M Bazzi
Apr 4, 2021·Microorganisms·Elena XuDespoina Koulenti
Apr 2, 2021·Journal of Clinical Microbiology·Salah GallahMarc Garnier
Jun 2, 2021·The Journal of Molecular Diagnostics : JMD·Zeshi LiuAili He
Aug 29, 2021·The Journal of Molecular Diagnostics : JMD·Yuanyuan BaiYan Jin