Mar 2, 2012

Diagnostic strategy for identifying avian pathogenic Escherichia coli based on four patterns of virulence genes

Journal of Clinical Microbiology
Catherine SchoulerMaryvonne Moulin-Schouleur

Abstract

In order to improve the identification of avian pathogenic Escherichia coli (APEC) strains, an extensive characterization of 1,491 E. coli isolates was conducted, based on serotyping, virulence genotyping, and experimental pathogenicity for chickens. The isolates originated from lesions of avian colibacillosis (n = 1,307) or from the intestines of healthy animals (n = 184) from France, Spain, and Belgium. A subset (460 isolates) of this collection was defined according to their virulence for chicks. Six serogroups (O1, O2, O5, O8, O18, and O78) accounted for 56.5% of the APEC isolates and 22.5% of the nonpathogenic isolates. Thirteen virulence genes were more frequently present in APEC isolates than in nonpathogenic isolates but, individually, none of them could allow the identification of an isolate as an APEC strain. In order to take into account the diversity of APEC strains, a statistical analysis based on a tree-modeling method was therefore conducted on the sample of 460 pathogenic and nonpathogenic isolates. This resulted in the identification of four different associations of virulence genes that enables the identification of 70.2% of the pathogenic strains. Pathogenic strains were identified with an error margin of 4.3...Continue Reading

  • References40
  • Citations38

References

  • References40
  • Citations38

Citations

Mentioned in this Paper

Alkalescens-Dispar Group
Pathogenicity
Pathogenic Organism
Escherichia Coli Infections
Serotyping
Serogroup
Isolate compound
Aves
Meleagridinae
Virulence