PMID: 9650964Jul 3, 1998Paper

Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples

Journal of Clinical Microbiology
J Helweg-LarsenB Lundgren

Abstract

There is a need to develop noninvasive methods for the diagnosis of Pneumocystis carinii pneumonia in patients unable to undergo bronchoscopy or induction sputum. Oral wash specimens are easily obtained, and P. carinii nucleic acid can be amplified and demonstrated by PCR. In routine clinical use, easy sample processing and single-round PCR are needed to ensure rapid analysis and to reduce the risk of contamination. We developed a single-round Touchdown PCR (TD-PCR) protocol with the ability to detect PCR inhibition in the specimen. The TD-PCR was evaluated in a routine diagnostic laboratory and was compared to a previously described PCR protocol (mitochondrial RNA) run in a research laboratory. Both PCR methods amplified a sequence of the mitochondrial rRNA gene of P. carinii. Paired bronchoalveolar lavage (BAL) and oral wash specimens from 76 consecutive human immunodeficiency virus type 1-infected persons undergoing a diagnostic bronchoscopy were included. The TD-PCR procedure was quicker than the mitochondrial PCR procedure (<24 versus 48 h) and, compared to microscopy, had sensitivity, specificity, and positive and negative predictive values of 89, 94, 93, and 91%, respectively, for oral wash specimens and 100, 91, 90, and...Continue Reading

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Citations

Jun 28, 2002·Comprehensive Therapy·Jean T SantamauroDiane E Stover
Nov 26, 2002·BMC Infectious Diseases·Jannik Helweg-LarsenBettina Lundgren
Aug 3, 2005·FEMS Immunology and Medical Microbiology·Isabelle Durand-JolyEduardo Dei-Cas
Oct 12, 2007·Paediatric Drugs·Sadhna M Shankar, Joseph J Nania
Apr 23, 2008·Current Opinion in Pulmonary Medicine·Bryan J KrajicekCharles F Thomas
Aug 26, 2010·Therapeutic Advances in Respiratory Disease·Eva M Carmona, Andrew H Limper
Jan 5, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J TorresC H Lee
Jul 24, 2001·Medical and Pediatric Oncology·A PoulsenK Schmiegelow
Jan 23, 2004·The Journal of Eukaryotic Microbiology·Laurence HuangCharles B Beard
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·David R Murdoch
Oct 13, 2006·Current Opinion in Infectious Diseases·R B Thomson
Jul 28, 2005·Pediatric Blood & Cancer·Merete Stubkjaer ChristensenHenrik Hasle
Oct 12, 2011·Current Opinion in Infectious Diseases·Alistair B ReidLeon J Worth
Feb 15, 2013·Journal of Clinical Microbiology·Kelvin K W ToKwok-Yung Yuen
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Oct 21, 2011·Journal of Clinical Microbiology·Yuan LuZouxiao Ying
Dec 16, 2006·The Journal of Eukaryotic Microbiology·Nieves RespaldizaCarmen De la Horra
Aug 24, 2016·The Journal of Antimicrobial Chemotherapy·Alexandre AlanioUNKNOWN 5th European Conference on Infections in Leukemia (ECIL-5), a joint venture of The European Group for Blood and Marrow Trans
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Nov 25, 2020·HIV Medicine·V SivarajR Kulasegaram
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Sep 28, 2001·Journal of Clinical Microbiology·J Helweg-LarsenJ D Lundgren
Aug 25, 2004·Clinics in Laboratory Medicine·Gary W Procop, Glenn D Roberts

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