Diagnostic value of an ELISA using a recombinant 54-kDa species-specific protein from Chlamydia pneumoniae

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
J-M SueurF Betsou

Abstract

The aim of this study was to evaluate a 54-kDa recombinant protein encoded by the CPn0980 gene for use in a Chlamydia pneumoniae-specific ELISA. The ability of this affinity-purified protein to detect C. pneumoniae-specific antibodies was evaluated with a panel of 105 serum samples from 62 patients with community-acquired pneumonia. The results of this assay were compared with those obtained with a direct PCR-based detection assay and an outer-membrane complex-based immunoassay. The 54-kDa protein induced specific antibodies following infection of humans, and the recombinant 54-kDa ELISA detected anti-C. pneumoniae IgG and/or IgM antibodies with a sensitivity of 66.7% and a specificity of 79.2% compared with the direct PCR-based detection assay.

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Citations

Jan 21, 2010·Journal of Clinical Laboratory Analysis·Chen HongliangWu Yimou
Aug 21, 2010·Journal of Medical Microbiology·Enrique VillegasJosé Gutiérrez
Mar 13, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J ZhengY Wu
Jan 31, 2012·Biochemical and Biophysical Research Communications·Sung-Ha ParkKwang Yeon Hwang

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