Diagonal-free 3D/4D HN,HN-TROSY-NOESY-TROSY

Journal of the American Chemical Society
Tammo DiercksOscar Millet

Abstract

Structural biology by NMR spectroscopy relies on measuring interproton distances via NOE cross-signals in nuclear Overhauser effect spectroscopy (NOESY) spectra. In proteins, the subset of H(N)-H'(N) NOE contacts is most important for deriving initial structural models and for spectral assignment by "NOE walking". Here we present a fully optimized NMR experiment for measuring these pivotal contacts: diagonal-free 3D/4D HN,HN-TROSY-NOESY-TROSY. It combines all of the critical requirements for extracting the optimal H(N)-H'(N) distance information: the highest resolution by consistent transverse relaxation-optimized spectroscopy (TROSY) evolution, the largest spectral dispersion in two (15)N dimensions, and maximal coverage and purity through specific suppression of the intense diagonal signals that are the main source of overlap, artifacts, and bias in any NOESY spectrum. Most notably, diagonal suppression here comes without compromising the NOE cross-signal intensities. This optimized experiment appears to be ideal for a broad range of structural studies, particularly on large deuterated, partially unfolded, helical, and membrane proteins.

References

Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·K V PervushinK Wüthrich
Sep 25, 1999·Journal of Magnetic Resonance·A Meissner, O W Sørensen
Apr 28, 2000·Journal of Magnetic Resonance·T Schulte-Herbrüggen, O W Sorensen
Jul 22, 2005·Journal of Biomolecular NMR·Tammo Diercks, Vladislav Y Orekhov
Aug 1, 1998·Journal of Biomolecular NMR·K V PervushinK Wüthrich

❮ Previous
Next ❯

Citations

May 10, 2013·Journal of Biomolecular NMR·Jan StanekWiktor Koźmiński
Aug 22, 2013·Journal of Biomolecular NMR·Jan StanekMirko Cevec
Oct 18, 2011·Journal of Molecular Biology·Markus MundRemco Sprangers
Nov 26, 2015·Progress in Nuclear Magnetic Resonance Spectroscopy·Michał NowakowskiWiktor Koźmiński
Mar 25, 2014·Journal of Magnetic Resonance·Jiří NováčekVladimír Sklenář
Jun 25, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Krzysztof KazimierczukWiktor Koźmiński
Jul 6, 2014·Journal of the American Chemical Society·Rasmus LinserGerhard Wagner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.