Abstract
A miniature fiber optic spectrometer enclosed within a semipermeable (dialysis) membrane is proposed for in vivo interstitial sensing applications. The semipermeable membrane acts as a molecular filter, allowing only small molecules to pass through to the sampling volume. This filtering, in principle, should enable continuous in vivo drug sensing, removing the necessity for complex microdialysis systems. We use a biological phantom to examine the reliable detection of a fluorescence signal from small dye molecules in the presence of large fluorophores and scatterers. We find that spectral artefacts arising from scatterers and large fluorophores are substantially suppressed, simplifying the spectral analysis. In addition, the measured sampling rate of 157 s is superior to existing in vivo tissue assaying techniques such as microdialysis, which can take tens of minutes.
References
Jan 1, 1991·Life Sciences·L StåhleU Ungerstedt
Sep 1, 1986·Acta Physiologica Scandinavica·U Tossman, U Ungerstedt
Oct 1, 1988·Journal of Neurochemistry·Y L HurdU Ungerstedt
Nov 1, 1986·Analytical Chemistry·O S Wolfbeis
Jan 1, 1984·Analytical Chemistry·J I PetersonD K Buckhold
Nov 1, 1996·Intensive Care Medicine·E E RoupieF J Lemaire
Jun 1, 1997·Photochemistry and Photobiology·C K BrooknerR R Richards-Kortum
Mar 25, 1998·Annals of the New York Academy of Sciences·S L Jacques
Oct 28, 1998·Biophysical Journal·R BrockT M Jovin
Sep 18, 1999·Analytical Chemistry·Y ZhaoP Pantano
Jan 1, 1997·Critical Care : the Official Journal of the Critical Care Forum·E E Roupie
Jul 20, 2001·British Journal of Cancer·J F ThompsonM S Roberts
Jan 25, 2003·Journal of Biomedical Optics·Urs Utzinger, Rebecca R Richards-Kortum
Oct 10, 1964·Nature·N S KAPANY, N SILBERTRUST
May 7, 2004·Physics in Medicine and Biology·Jeremy C HebdenJohn S Wyatt
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Robert SauermannChristian Joukhadar
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Suriani Abdul RaniPhilip S Stewart