PMID: 11340109May 8, 2001Paper

Diarrhea reduces the rates of cardiac protein synthesis in myofibrillar protein fractions in rats in vivo

The Journal of Nutrition
R J HunterV R Preedy

Abstract

Although chronic diarrhea affects heart function and morphology, the pathogenic mechanisms are unknown. It was our hypothesis that diarrhea imposes metabolic stress to inhibit the synthesis of new contractile proteins. To test this hypothesis, we investigated the effects of lactose-induced diarrhea in rats. The groups were: 1) freely fed controls, 2) rats with lactose-induced diarrhea or 3) pair-fed rats. After 1 wk, hearts from the rats were subjected to subcellular fractionation techniques to isolate the major protein fractions, including myofibrillar proteins. The rates of protein synthesis were measured with concomitant assay of cardiac composition and plasma analytes. In comparison with the control group, diarrhea induced the following changes (P < 0.05): a decrease in heart weight, reduced RNA and mixed protein contents and a reduction in the fractional rate of mixed protein synthesis. There was a reduction in the content of all protein fractions. The fractional synthesis rate was reduced only for the myofibrillar fraction. Plasma insulin-like growth factor-I, but not corticosterone, was reduced. Plasma cholesterol and triglyceride concentrations were also reduced. In comparison with the pair-fed group, diarrhea induced t...Continue Reading

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Citations

Jan 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eileen M Crimmins, Caleb E Finch
Dec 2, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·J Marc RhoadsJack Odle
Jul 17, 2015·Journal of Cardiovascular Magnetic Resonance : Official Journal of the Society for Cardiovascular Magnetic Resonance·Russell DibbChunlei Liu
Apr 4, 2003·The Journal of Nutrition·R J HunterV R Preedy
May 21, 2016·NMR in Biomedicine·Russell DibbChunlei Liu
Jan 14, 2009·International Journal of Cardiology·Olga V AnatskayaAlexander E Vinogradov
Apr 29, 2009·Journal of Molecular and Cellular Cardiology·William T FestucciaYves Deshaies
Sep 18, 2004·Science·Caleb E Finch, Eileen M Crimmins

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