Diazaborine treatment of yeast cells inhibits maturation of the 60S ribosomal subunit

Molecular and Cellular Biology
Brigitte PertschyHelmut Bergler

Abstract

Diazaborine treatment of yeast cells was shown previously to cause accumulation of aberrant, 3'-elongated mRNAs. Here we demonstrate that the drug inhibits maturation of rRNAs for the large ribosomal subunit. Pulse-chase analyses showed that the processing of the 27S pre-rRNA to consecutive species was blocked in the drug-treated wild-type strain. The steady-state level of the 7S pre-rRNA was clearly reduced after short-term treatment with the inhibitor. At the same time an increase of the 35S pre-rRNA was observed. Longer incubation with the inhibitor resulted in a decrease of the 27S precursor. Primer extension assays showed that an early step in 27S pre-rRNA processing is inhibited, which results in an accumulation of the 27SA2 pre-rRNA and a strong decrease of the 27SA3, 27SB1L, and 27SB1S precursors. The rRNA processing pattern observed after diazaborine treatment resembles that reported after depletion of the RNA binding protein Nop4p/Nop77p. This protein is essential for correct pre-27S rRNA processing. Using a green fluorescent protein-Nop4 fusion, we found that diazaborine treatment causes, within minutes, a rapid redistribution of the protein from the nucleolus to the periphery of the nucleus, which provides a possibl...Continue Reading

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Citations

Jan 20, 2009·Cellular and Molecular Life Sciences : CMLS·R P Massengo-Tiassé, J E Cronan
Jul 25, 2007·Molecular and Cellular Biology·Brigitte PertschyHelmut Bergler
Nov 18, 2015·Nature Chemical Biology·Jonathan M Stokes, Eric D Brown
Jan 3, 2018·Nucleic Acids Research·Gertrude ZisserHelmut Bergler
Feb 14, 2015·Chembiochem : a European Journal of Chemical Biology·Kara FinzelMichael D Burkart
Nov 13, 2013·Molecular Systems Biology·Fabien MorettoBenoît Pinson
Mar 8, 2019·ELife·Blake W TyeL Stirling Churchman
Nov 11, 2019·Biomolecules·Michael PrattesRobin E Stanley
Feb 3, 2005·Yeast
Jun 12, 2019·BMC Biology·Dominik AwadHelmut Bergler
Apr 29, 2020·International Journal of Molecular Sciences·Lisa KoflerHelmut Bergler
Dec 29, 2013·The Journal of Biological Chemistry·Mathias LoiblHelmut Bergler
Jun 11, 2021·Nature Communications·Michael PrattesHelmut Bergler
Nov 2, 2021·Organic Letters·Pinwen CaiDennis Gillingham

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