Diazirine photocrosslinking recruits activated FTO demethylase complexes for specific N(6)-methyladenosine recognition.

ACS Chemical Biology
Hyun Seok JeongAkimitsu Okamoto

Abstract

N(6)-methyladenosine (m(6)A) is a prevalent modification of RNAs. m(6)A exists in mRNA and plays an important role in RNA biological pathways and in RNA epigenetic regulation. We applied diazirine photocrosslinking to the event of m(6)A recognition mediated by the fat mass and obesity associated (FTO) demethylase. A highly photoreactive diazirine adjacent to m(6)A on the RNA successfully recruited activated FTO complexes with an m(6)A preference. The process of recognition of m(6)A via FTO using diazirine photocrosslinking was controlled by the α-ketoglutarate (α-KG) cosubstrate and the Fe(II) cofactor, which are involved in m(6)A oxidative demethylation. In addition, FTO bound to ssRNAs prior to the m(6)A recognition process. Diazirine photocrosslinking contributes to increasing the chances of capturing activated FTO complexes with specific m(6)A recognition and provides new insights into the dynamic FTO oxidative demethylation process.

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P S PendergrastR H Ebright
May 26, 2004·Chemistry & Biology·Lyle Burdine, Thomas Kodadek
Nov 18, 2008·Chembiochem : a European Journal of Chemical Biology·Malte WinnackerThomas Carell
Oct 18, 2011·Nature Chemical Biology·Guifang JiaChuan He
Oct 3, 2012·Bioorganic & Medicinal Chemistry·David J Lapinsky
Oct 11, 2012·Journal of the American Chemical Society·Baoen ChenCai-Guang Yang
Oct 25, 2012·Current Opinion in Chemical Biology·Ye Fu, Chuan He
Dec 12, 2012·Trends in Genetics : TIG·Guifang JiaChuan He
Jan 23, 2013·Trends in Biochemical Sciences·Tao Pan
Apr 4, 2013·Journal of Medicinal Chemistry·WeiShen AikChristopher J Schofield
Dec 1, 2009·Current Protocols in Chemical Biology·Andrew L Mackinnon, Jack Taunton
Sep 3, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jitapa Sumranjit, Sang J Chung
Dec 10, 2013·Nature Chemical Biology·Jianzhao LiuChuan He
Mar 26, 2014·Nature Reviews. Genetics·Ye FuChuan He
Apr 2, 2014·Chemical Communications : Chem Comm·Huimin ZhangChaoyong James Yang
Apr 10, 2014·Nature Reviews. Molecular Cell Biology·Kate D Meyer, Samie R Jaffrey

❮ Previous
Next ❯

Citations

Oct 29, 2019·Chembiochem : a European Journal of Chemical Biology·Dmytro DziubaCarsten Schultz
Jul 1, 2020·Chemistry : a European Journal·Jessica Rodríguez, Miguel Martínez-Calvo
Oct 8, 2020·Chemical Communications : Chem Comm·Jerrin Thomas George, Seergazhi G Srivatsan
Jan 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shifali Shishodia, Christopher J Schofield
Mar 19, 2021·Clinical and Experimental Pharmacology & Physiology·Zunera ChauhdaryMuhammad Sajid Hamid Akash

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.