Diazoxide attenuates ischemia/reperfusion injury via upregulation of heme oxygenase-1 after liver transplantation in rats.

World Journal of Gastroenterology : WJG
Zhong ZengMing-Qing Chen

Abstract

To evaluate the effects of diazoxide on ischemia/reperfusion (I/R)-injured hepatocytes and further elucidate its underlying mechanisms. Male Sprague-Dawley rats were randomized (8 for donor and recipient per group) into five groups: I/R group (4 h of liver cold ischemia followed by 6 h of reperfusion); heme oxygenase-1 (HO-1) small interfering RNA (siRNA) group (injection of siRNA via donor portal vein 48 h prior to harvest); diazoxide (DZ) group (injection of DZ via donor portal vein 10 min prior to harvest); HO-1 siRNA + DZ group; and siRNA control group. Blood and liver samples were collected at 6 h after reperfusion. The mRNA expressions and protein levels of HO-1 were determined by reverse transcription polymerase chain reaction and Western blotting, and tissue morphology was examined by light and transmission electron microscopy. Serum transaminases level and cytokines concentration were also measured. We observed that a significant reduction of HO-1 mRNA and protein levels in HO-1 siRNA and HO-1 siRNA + DZ group when compared with I/R group, while the increases were prominent in the DZ group. Light and transmission electron microscopy indicated severe disruption of tissue with lobular distortion and mitochondrial cristae...Continue Reading

References

Jan 1, 1997·Annual Review of Pharmacology and Toxicology·M D Maines
Feb 15, 2001·Brain Research·G DebskaW S Kunz
Aug 6, 2002·Cardiovascular Research·Olaf OldenburgJames M Downey
Oct 23, 2002·Archives of Histology and Cytology·Takashi KobayashiMakoto Naito
Oct 26, 2002·Free Radical Biology & Medicine·Juan C CutrnGiuseppe Poli
Dec 4, 2002·Biochimica Et Biophysica Acta·Grazyna DebskaWolfram S Kunz
Feb 15, 2003·FEBS Letters·Renato FerrantiAlicia J Kowaltowski
Mar 29, 2003·Journal of Hepatology·Alexandra K KiemerAngelika M Vollmar
Dec 23, 2003·The Journal of Biological Chemistry·Xuchen ZhangPatty J Lee
Sep 4, 2004·Antioxidants & Redox Signaling·Mahin D Maines
Jul 6, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Erwin GeukenRobert J Porte
Feb 8, 2006·American Journal of Physiology. Renal Physiology·Kristin A Kirkby, Christopher A Adin
Apr 4, 2007·Annals of the New York Academy of Sciences·Gábor JancsóErzsébet Róth
Sep 8, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Fabienne TamionChristian Thuillez
Jun 13, 2008·The Journal of Surgical Research·Peter ThomasR Armour Forse
Nov 13, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Luke DeveyStephen J Wigmore
Feb 4, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Jung-Woo BaeSeok-Yong Lee
Mar 12, 2010·World Journal of Gastroenterology : WJG·Zhong ZengYu-Jun Zhang

❮ Previous
Next ❯

Citations

Apr 23, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Xiao-Qing ChenYan-Rong Tang
Jan 23, 2016·Oxidative Medicine and Cellular Longevity·Guangyin ZhangJunping Zhang
Nov 26, 2013·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Elena GrossiniGiovanni Vacca

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.