Dibenzoylmethane exerts metabolic activity through regulation of AMP-activated protein kinase (AMPK)-mediated glucose uptake and adipogenesis pathways

PloS One
Nami KimHyeon Soo Kim

Abstract

Dibenzoylmethane (DBM) has been shown to exert a variety of beneficial effects on human health. However, the mechanism of action is poorly understood. In this study, DBM increased phosphorylation of AMP-activated protein kinase (AMPK) and stimulated glucose uptake in a skeletal muscle cell line. Both knockdown of AMPK with siRNA and inhibition with AMPK inhibitor blocked DBM-induced glucose uptake. DBM increased the concentration of intracellular calcium and glucose uptake due to DBM was abolished by STO-609 (a calcium/calmodulin-dependent protein kinase inhibitor). DBM stimulated phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), which was blocked by pretreatment with compound C, an AMPK inhibitor. The expression of glucose transporter type 4 (GLUT4) was increased by DBM. The translocation of GLUT4 to the plasma membrane was also increased by DBM in AMPK dependently. In addition, DBM suppressed weight gain and prevented fat accumulation in the liver and abdomen in mice fed a high-fat diet. In pre-adipocyte cells, DBM decreased the activity of acetyl-CoA carboxylase (ACC), the rate-limiting enzyme of fatty acid synthesis. Expression of the adipogenic gene, fatty acid synthase (FAS), was suppressed by DBM in an ...Continue Reading

References

Aug 1, 1978·Acta Pharmacologica Et Toxicologica·B Wahlström, G Blennow
Feb 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·V SarabiaA Klip
Oct 6, 1998·Annual Review of Biochemistry·D G HardieM Carlson
Dec 18, 1998·The Journal of Biological Chemistry·B E CruteL A Witters
Feb 28, 2002·Cancer Letters·Kimberly M JacksonWayne B Harris
Jun 28, 2003·FEBS Letters·D Grahame HardieEmma R Hudson
Nov 15, 2003·Current Biology : CB·Angela WoodsDavid Carling
Oct 29, 2004·Journal of Cell Science·D Grahame Hardie
Jun 7, 2005·Journal of the American Society of Nephrology : JASN·Jean-François Yale
Jun 28, 2005·The Journal of Biological Chemistry·Rebecca L HurleyLee A Witters
Jan 19, 2006·American Journal of Physiology. Endocrinology and Metabolism·Nadeeja WijesekaraAmira Klip
Mar 6, 2010·Journal of Cellular Physiology·Ji Hae KimHyeon Soo Kim
May 7, 2013·Molecular Cancer·Carla RozzoMarina Pisano
Nov 28, 2013·Current Cancer Drug Targets·Adi ZhengJiankang Liu

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Citations

Jul 11, 2018·Wiley Interdisciplinary Reviews. RNA·Graham D Pavitt
Nov 27, 2018·The Journal of Endocrinology·Eun Soo LeeChoon Hee Chung
Aug 18, 2020·Journal of Clinical Biochemistry and Nutrition·Hitoshi AshidaYoko Yamashita
Mar 4, 2021·Biomolecules·Talya ShachamGerardo Z Lederkremer

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