Dibutyryl cAMP influences endothelial progenitor cell recruitment during wound neovascularization

The Journal of Investigative Dermatology
Jun AsaiSaburo Kishimoto

Abstract

Delayed wound healing is one of the major complications of diabetes, and is caused by delayed cellular infiltration, reduced angiogenesis, and decreased formation and organization of collagen fibers. Recently, endothelial progenitor cells (EPC) isolated from peripheral blood were shown to accumulate at sites of neovascularization during wound healing. The present study tested the hypothesis that sodium N-6,2'-O-dibutyryl adenosine-3',5'-cyclic phosphate (DBcAMP), which has been shown to accelerate wound healing, promotes recruitment of EPC into wounds and contributes to the stimulation of neovascularization in genetically diabetic mice. Topical application of DBcAMP resulted in significant acceleration of wound healing and wound vascularization partly via enhanced recruitment of EPC. EPC in DBcAMP-treated wounds were mainly localized to cell clusters at the border of the granulation tissue, a site where blood supply is most insufficient. DBcAMP treatment increased the mRNA expression of angiogenic cytokines vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1alpha (SDF-1alpha) in vivo in wound tissue and in cultured fibroblasts and macrophages, in vitro. Culture supernatants of DBcAMP-treated cells enhanc...Continue Reading

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Citations

Oct 18, 2012·Annual Review of Pharmacology and Toxicology·Satish CheepalaJohn D Schuetz
Feb 5, 2013·Biochemical Pharmacology·Kim H T Paraiso, Keiran S M Smalley
Nov 4, 2011·The Journal of Investigative Dermatology·Yukihide NishimuraDouglas W Losordo
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Feb 23, 2010·Journal of Neuroscience Research·Hwa Kyoung ShinChi Dae Kim
Nov 9, 2016·Prostaglandins & Other Lipid Mediators·Elizabeth BerryAustin M Guo
Apr 6, 2007·Journal of the American Society of Nephrology : JASN·Daniel PatschanMichael S Goligorsky
Jun 26, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·O SimonettiA Scaloni
Nov 30, 2019·Cells·Mara MassimiMauro Giorgi
Apr 29, 2010·Circulation Journal : Official Journal of the Japanese Circulation Society·Jun-ichi KawabeNaoyuki Hasebe
Jun 10, 2021·Journal of Central Nervous System Disease·Norihito FukawaJun Takahashi

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