Dibutyryl cyclic adenosine monophosphate restores the ability of aged Leydig cells to produce testosterone at the high levels characteristic of young cells

Endocrinology
Haolin ChenBarry R Zirkin

Abstract

The wealth of knowledge about the function and regulation of adult Leydig cells, the cells within the mammalian testis that produce testosterone, make these cells ideal for studying principles and mechanisms of aging. A hallmark of mammalian aging is decreased serum testosterone concentration. In the Brown Norway rat, this has been shown to be associated with the reduced ability of aged Leydig cells to produce testosterone in response to LH. Herein, we demonstrate that culturing the aged cells with dibutyryl cAMP, a membrane-permeable cAMP agonist that bypasses the LH receptor-adenlyly cyclase cascade, restores testosterone production to levels comparable to those of young cells and also restores steroidogenic acute regulatory protein and P450scc, the proteins involved in the rate-limiting steps of steroidogenesis. These results strongly suggest that signal transduction deficits are responsible for reduced steroidogenesis by aged Leydig cells and that bypassing signal transduction reverses the steroidogenic decline by the aged cells.

References

Jul 1, 1979·Physiological Reviews·B ChanceA Boveris
Jan 15, 1979·Analytical Biochemistry·C F BrunkT W James
Mar 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G R Klinefelter, L L Ewing
Jan 1, 1986·Molecular and Cellular Endocrinology·J KolenaE Seböková
Nov 1, 1973·Journal of Reproduction and Fertility·L G StrattonC Desjardins
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M L DufauK J Catt
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·C CascioV Papadopoulos
Jun 26, 1999·The Journal of Biological Chemistry·M McVeyC Klein
Oct 26, 1999·Experimental Gerontology·M HashimotoS Masumura
Dec 13, 2000·Experimental Gerontology·J D Veldhuis
Apr 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Christina Wang, Ronald S Swerdloff

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Citations

Sep 19, 2007·Annals of Nuclear Medicine·Kazuhiro KitajimaKazuro Sugimura
Jun 9, 2005·Molecular and Cellular Endocrinology·XingJia Wang, Douglas M Stocco
Jul 16, 2014·Experimental Gerontology·S J SokanovicT S Kostic
Sep 2, 2008·Molecular and Cellular Endocrinology·Andrew S MidzakBarry R Zirkin
Aug 2, 2005·Experimental Gerontology·Haolin ChenBarry R Zirkin
Feb 24, 2015·Experimental Gerontology·M C BeattieB R Zirkin
Jan 25, 2013·Nutrition Research and Practice·Yoo-Hun NohSoon Chul Myung
Nov 9, 2016·World Journal of Stem Cells·Taylor C PeakWayne J Hellstrom
Jul 28, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Yiyan WangHaolin Chen
Aug 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michael CurleyLee B Smith
Feb 25, 2006·Human Reproduction·V RouletN Dejucq-Rainsford
Sep 18, 2004·Endocrinology·Matthew P Hardy, Peter N Schlegel
Dec 5, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Aleksandar Z BaburskiTatjana S Kostic

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