Abstract
Epithelial barrier dysfunction is associated with a number of inflammatory disorders. However, the pathogenesis of epithelial barrier dysfunction is unclear. This study aims to elucidate the involvement of dicaine in airway epithelial barrier dysfunction. In the present study, an RPMI2650 (Rpc) human airway epithelial cell line was cultured, with or without dicaine, in monolayers using Transwells. In order to assess airway epithelial barrier function, the levels of transepithelial electrical resistance and permeability to ovalbumin (OVA) were measured. Expression of apoptosis-linked gene 2-interacting protein X (Alix) in Rpc cells was assessed using quantitative reverse transcription‑polymerase chain reaction and western blotting. The antigenicity of OVA was assessed using a T cell proliferation assay. The results of the present study demonstrated that Alix expression levels were markedly lower in Rpc cells treated with dicaine, compared with those not treated with dicaine. An increase in the level of transcellular permeability to OVA was observed in Rpc monolayers following treatment with dicaine, compared with that in the Rpc monolayer without dicaine treatment. Furthermore, the Rpc monolayer maintained high antigenicity and ...Continue Reading
References
Oct 6, 2000·The Journal of Clinical Investigation·P C YangM H Perdue
Aug 7, 2001·Gastroenterology·L C YuM H Perdue
Mar 15, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Linda C H YuMary H Perdue
Oct 31, 2003·The Journal of Biological Chemistry·Yaël TrioulierRémy Sadoul
Jan 10, 2006·The American Journal of Pathology·Ping-Chang YangMary H Perdue
Sep 1, 2006·Immunology and Cell Biology·Tao LiuPing-Chang Yang
Mar 14, 2007·Cell·Robert D FisherChristopher P Hill
Dec 11, 2007·Nature Structural & Molecular Biology·Qianting ZhaiChristopher P Hill
Jan 16, 2009·Biochemical Society Transactions·Anne-Laure Mahul-MellierRémy Sadoul
Jan 27, 2009·Journal of Proteome Research·Micha HoffmannDirk Haller
Aug 5, 2009·Gut·P-Y ZhengP-C Yang
May 18, 2010·Biochemical and Biophysical Research Communications·Flavie StrappazzonRémy Sadoul
Jun 8, 2012·Annals of the New York Academy of Sciences·Declan F McCole
Jul 12, 2012·Cellular and Molecular Life Sciences : CMLS·Takuya Suzuki
Sep 7, 2012·The Journal of Investigative Dermatology·Lisa M SevillaPaloma Pérez
Jul 11, 2013·PloS One·Yong YuPing-Chang Yang
Sep 7, 2013·PloS One·Marina S LeinoDonna E Davies
Mar 26, 2014·Tissue Barriers·Bahman SaatianSteve N Georas
Apr 10, 2014·Journal of Biomedical Science·Hao YanZhigang Liu