Dicentric assay: inter-laboratory comparison in Indian laboratories for routine and triage applications

Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine
D BakkiamB Venkatraman

Abstract

An Inter-Laboratory Comparison (ILC) study on Dicentric Chromosome Assay (DCA) was carried out between two Indian biodosimetry labs. Human peripheral blood samples exposed to 10 different doses of X-rays up to 5Gy were shared between the labs to generate calibration data. Validation of calibration curves was done by dose estimation of coded samples exposed to X- or gamma radiation. Reliability of the DCA data for triage application was evaluated by scoring 20, 50 and 100 metaphases in the dose range of 0.5-3.0Gy. No significant difference was observed between labs regarding the established calibration data as well as the DCA triage dose assessments. Scoring of 20 metaphases (MP) was adequate to detect radiation exposure of >2Gy whereas 50 MP were sufficient to determine exposures of 0.5Gy. Both labs performed the DCA in a reliable manner and made the first step in setting up a biodosimetry network in India.

References

Jun 3, 2000·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·D C LloydY C Guerrero-Carbajal
Dec 13, 2007·Radiation Protection Dosimetry·Joanna DeperasAndrzej Wojcik
Jul 19, 2013·Radiation Research·C BeinkeM Abend
Jul 12, 2014·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·M BhavaniN K Chaudhury

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Citations

Aug 26, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Raavi VenkateswarluPerumal Venkatachalam
Jul 15, 2016·Radiation Protection Dosimetry·Horst RommElizabeth A Ainsbury
Jan 23, 2018·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Akshaykumar A NayakSatish Rao
Sep 25, 2018·Radiation Protection Dosimetry·Adayabalam S BalajeeNicholas Dainiak
Feb 7, 2019·Radiation Protection Dosimetry·Nicholas DainiakWilliam F Blakely
Feb 16, 2018·Journal of Radiological Protection : Official Journal of the Society for Radiological Protection·Akshaykumar A NayakB S Satish Rao
Oct 29, 2017·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Bhavani ManivannanVenkatachalam Perumal
May 11, 2021·International Journal of Radiation Biology·Eric GregoireUlrike Kulka

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