Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize.

Nature Communications
C. TengVirginia Walbot

Abstract

Small RNAs play important roles during plant development by regulating transcript levels of target mRNAs, maintaining genome integrity, and reinforcing DNA methylation. Dicer-like 5 (Dcl5) is proposed to be responsible for precise slicing in many monocots to generate diverse 24-nt phased, secondary small interfering RNAs (phasiRNAs), which are exceptionally abundant in meiotic anthers of diverse flowering plants. The importance and functions of these phasiRNAs remain unclear. Here, we characterized several mutants of dcl5, including alleles generated by the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system and a transposon-disrupted allele. We report that dcl5 mutants have few or no 24-nt phasiRNAs, develop short anthers with defective tapetal cells, and exhibit temperature-sensitive male fertility. We propose that DCL5 and 24-nt phasiRNAs are critical for fertility under growth regimes for optimal yield.

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Citations

Jan 26, 2021·Plant Reproduction·Stefanie Dukowic-Schulze, Karina van der Linde
Jan 30, 2021·Frontiers in Plant Science·Abdellah BarakateRobbie Waugh
Jan 6, 2021·Nature Plants·Yang-Seok LeeJose Gutierrez-Marcos
Mar 3, 2021·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Yunfei LiWeiwei Jin
Jun 24, 2021·The Plant Journal : for Cell and Molecular Biology·Suresh PokhrelBlake C Meyers
Oct 22, 2021·Frontiers in Plant Science·Arun S SeetharamMatthew B Hufford
Nov 12, 2021·Genes & Genetic Systems·Reina Komiya
Jan 13, 2022·The Plant Cell·Guo-Ling NanVirginia Walbot

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Methods Mentioned

BETA
confocal microscopy
fluorescence microscopy
Transmission
RNA-seq
PCR
electrophoresis
sRNA-seq

Software Mentioned

PlantCV
Tophat
R
Trimmomatic
DEseq2
Amira
Bowtie
CRISPR Genome Analysis Tool
Cufflinks
FeatureCounts

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