Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic Fungus Fusarium graminearum

Frontiers in Microbiology
Wenping ZengJiasen Cheng

Abstract

Ascospores act as the primary inoculum of Fusarium graminearum, which causes the destructive disease Fusarium head blight (FHB), or scab. MicroRNAs (miRNAs) have been reported in the F. graminearum vegetative stage, and Fgdcl2 is involved in microRNA-like RNA (milRNA) biogenesis but has no major impact on vegetative growth, abiotic stress or pathogenesis. In the present study, we found that ascospore discharge was decreased in the Fgdcl1 deletion mutant, and completely blocked in the double-deletion mutant of Fgdcl1 and Fgdcl2. Besides, more immature asci were observed in the double-deletion mutant. Interestingly, the up-regulated differentially expressed genes (DEGs) common to ΔFgdcl1 and ΔFgdcl1/2 were related to ion transmembrane transporter and membrane components. The combination of small RNA and transcriptome sequencing with bioinformatics analysis predicted 143 novel milRNAs in wild-type perithecia, and 138 of these milRNAs partly or absolutely depended on Fgdcl1, while only 5 novel milRNAs were still obtained in the Fgdcl1 and Fgdcl2 double-deletion mutant. Furthermore, 117 potential target genes were predicted. Overall, Fgdcl1 and Fgdcl2 genes were partly functionally redundant in ascospore discharge and perithecium-sp...Continue Reading

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Citations

Mar 10, 2019·The New Phytologist·Jérôme CollemareMarc-Henri Lebrun
Mar 30, 2020·The New Phytologist·Ming XuLili Huang
Jun 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shuang WeiLiying Sun
Jan 24, 2020·Frontiers in Plant Science·Xin MaJavier Palma-Guerrero
Jul 25, 2020·Functional & Integrative Genomics·Himanshu DubeyTilak Raj Sharma
Apr 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shuang WeiLiying Sun
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ruiling BianLiying Sun
May 16, 2020·Frontiers in Plant Science·Bernhard Timo WernerAline Michaela Koch
Dec 12, 2020·International Journal of Molecular Sciences·Carlos LaxSilvia Calo
Jun 3, 2021·Annual Review of Phytopathology·Yongli QiaoWenbo Ma

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Methods Mentioned

BETA
PCR
RNA-seq
environmental stress
dissection
light microscopy

Software Mentioned

bowtie
REVIGO
FeatureCounts
MIREAP
psRobot
FASTX
TargetFinder
BLAST
Ensembl
edgeRun

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