Dicer regulates Nosema ceranae proliferation in honeybees

Insect Molecular Biology
Q HuangJ D Evans

Abstract

Nosema ceranae is a microsporidian parasite that infects the honeybee midgut epithelium. The protein-coding gene Dicer is lost in most microsporidian genomes but is present in N. ceranae. By feeding infected honeybees with small interfering RNA targeting the N. ceranae gene coding Dicer (siRNA-Dicer), we found that N. ceranae spore loads were significantly reduced. In addition, over 10% of total parasite protein-coding genes showed significantly divergent expression profiles after siRNA-Dicer treatment. Parasite genes for cell proliferation, ABC transporters and hexokinase were downregulated at 3 days postinfection, a key point in the middle of parasite replication cycles. In addition, genes involved in metabolic pathways of honeybees and N. ceranae showed significant co-expression. Furthermore, the siRNA-Dicer treatment partly reversed the expression patterns of honeybee genes. The honeybee gene mucin-2-like showed significantly upregulation in the siRNA-Dicer group compared with the infection group continually at 4, 5 and 6 days postinfection, suggesting that the siRNA-Dicer feeding promoted the strength of the mucus barrier resulted from interrupted parasite proliferation. As the gene Dicer broadly regulates N. ceranae proli...Continue Reading

References

Jan 1, 1995·Annual Review of Physiology·S J Gendler, A P Spicer
Feb 1, 1997·Molecular Microbiology·W A HanlonS Inouye
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Nov 29, 2002·Nature Genetics·Mark E J WoolhouseBruce R Levin
Feb 7, 2003·Current Medicinal Chemistry·Michaela ScherrMatthias Eder
Jan 13, 2004·Oncogene·Andrew FeberColin S Cooper
Jul 12, 2005·Folia Parasitologica·Jirí Lom, Iva Dyková
Jul 12, 2005·International Journal for Parasitology·Yanji Xu, Louis M Weiss
Feb 24, 2006·Journal of Cell Science·Ing Swie GopingR Chris Bleackley
Jun 7, 2006·International Journal for Parasitology·C del AguilaY Revilla
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Martin E LidellGunnar C Hansson
Oct 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lena BurriPatrick J Keeling
Jan 16, 2007·Journal of Invertebrate Pathology·Mariano HigesAránzazu Meana
Nov 4, 2008·Current Biology : CB·Soo Chan LeeJoseph Heitman
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
Jan 13, 2009·Journal of Invertebrate Pathology·Christopher Mayack, Dhruba Naug
Jul 14, 2010·Applied and Environmental Microbiology·Nitzan PaldiJay D Evans
Mar 17, 2011·Nature Reviews. Microbiology·Michael A McGuckinTimothy H Florin
May 10, 2011·International Journal of Medical Microbiology : IJMM·Wolfgang BohneUwe Gross
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jean-François PombertPatrick J Keeling
Oct 17, 2012·PLoS Neglected Tropical Diseases·Sumaira Z HasnainDavid J Thornton
Nov 20, 2012·Nucleic Acids Research·Hai Fang, Julian Gough
Jan 1, 2013·Nucleic Acids Research·Adam James Reid, Matthew Berriman
Jul 19, 2013·Environmental Microbiology Reports·Mariano HigesRaquel Martín-Hernández
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Seth M BarribeauPaul Schmid-Hempel
Sep 23, 2014·Applied and Environmental Microbiology·J Elijah PowellNancy A Moran
Jan 1, 2013·Pathogens·Janice J Kim, Waliul I Khan
Jul 19, 2015·Acta Biochimica Et Biophysica Sinica·Xinyi HeXingmeng Lu
Mar 15, 2016·Science Advances·Jomar F RabajanteDieter Ebert

❮ Previous
Next ❯

Citations

Sep 10, 2020·Archives of Insect Biochemistry and Physiology·In-Hui KimSoo-Dong Woo
May 23, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shan Shan ShaoQiang Huang
Jun 22, 2021·Frontiers in Microbiology·Qiang HuangJay D Evans

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved