Dicer1-mediated miRNA processing shapes the mRNA profile and function of murine platelets.

Blood
Jesse W RowleyAndrew S Weyrich

Abstract

Human platelets contain microRNAs (miRNAs) and miRNA processing machinery, but their contribution to platelet function remains incompletely understood. Here, we show that murine megakaryocyte (MK)-specific knockdown of Dicer1, the ribonuclease that cleaves miRNA precursors into mature miRNAs, reduces the level of the majority of miRNAs in platelets. This leads to altered platelet messenger RNA (mRNA) expression profiles and mild thrombocytopenia. Fibrinogen receptor subunits Itga2b (αIIb) and Itgb3 (β3) mRNAs were among the differentially expressed transcripts that are increased in platelets lacking Dicer1. Argonaute 2 (Ago2), a member of the miRNA silencing complex, co-immunoprecipitated with αIIband β3mRNAs in wild-type platelets. Furthermore, co-immunoprecipitation experiments suggested reduced αIIb/β3/Ago2 complexes in miRNA-deficient platelets. These results suggested that miRNAs regulate both integrin subunits. Subsequent 3' untranslated region luciferase reporter assays confirmed that the translation of both αIIband β3mRNAs can be regulated by miRNAs miR-326, miR-128, miR-331, and miR-500. Consistent with these molecular changes, the deletion ofDicer1resulted in increased surface expression of integrins αIIband β3, and e...Continue Reading

Associated Clinical Trials

References

May 1, 1981·British Journal of Haematology·C Giles
Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Oct 27, 2001·Science·R C Lee, V Ambros
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Oct 7, 2003·Nature Genetics·Emily BernsteinGregory J Hannon
Sep 17, 2004·The EMBO Journal·Yoontae LeeV Narry Kim
Jul 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian D HarfeClifford J Tabin
Aug 24, 2007·Transfusion·Jonathan N Thon, Dana V Devine
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Trinna L CuellarMichael T McManus
May 10, 2008·Bioinformatics·Pan DuSimon M Lin
May 30, 2008·Haematologica·Hana BruchovaJosef T Prchal
Jun 25, 2008·European Journal of Haematology·Michaela MerkerovaHana Bruchova
Aug 1, 2008·Nature·Daehyun BaekDavid P Bartel
Aug 30, 2008·The Journal of Experimental Medicine·Mark M W ChongDan R Littman
Jan 30, 2009·RNA·Sylvain PradervandKeith Harshman
Aug 12, 2009·Nature Structural & Molecular Biology·Patricia LandryPatrick Provost
Dec 1, 2009·Journal of Thrombosis and Haemostasis : JTH·A A KondkarP F Bray
Mar 3, 2011·Blood·Leonard C Edelstein, Paul F Bray
Apr 30, 2011·Methods in Molecular Biology·Marjorie P PerronPatrick Provost
May 21, 2011·Blood·Jesse W RowleyAndrew S Weyrich
Aug 31, 2011·Molecular Cancer Research : MCR·Katarzyna AugoffKhalid Sossey-Alaoui
Sep 14, 2011·British Journal of Haematology·Huiyuan LiRenchi Yang
Sep 15, 2011·Nucleic Acids Research·Christopher R SibleyMatthew J A Wood
Mar 15, 2013·RNA·Natalia J Martinez, Richard I Gregory
Jul 17, 2013·Journal of Thrombosis and Haemostasis : JTH·L C EdelsteinP F Bray
Jul 19, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Weiguo ChenJeffrey R Jacobson
Jul 26, 2014·The Journal of Clinical Investigation·Dallas S ShiAndrew S Weyrich
Apr 24, 2015·Thrombosis and Haemostasis·Giovanni CimminoPaolo Golino
May 7, 2015·Circulation Research·Amro ElgheznawyIngrid Fleming

❮ Previous
Next ❯

Citations

Jul 8, 2016·Thrombosis and Haemostasis·Rory R Koenen
Nov 7, 2016·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Yuzhong YanRong Xia
Feb 19, 2017·Blood·Eric W MillsNicholas T Ingolia
Jul 16, 2016·International Journal of Molecular Sciences·Cécile OuryPatrizio Lancellotti
Aug 11, 2017·Antioxidants & Redox Signaling·Amro Elgheznawy, Ingrid Fleming
Mar 27, 2018·Journal of Thrombosis and Haemostasis : JTH·B K ManneM T Rondina
Nov 24, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert A CampbellMatthew T Rondina
May 23, 2018·Platelets·Ascensión M De Los Reyes-GarcíaConstantino Martínez
May 20, 2018·British Journal of Pharmacology·Raphael LeblancOlivier Peyruchaud
Apr 9, 2016·Blood·Eric Boilard, Clémence Belleannée
Dec 30, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Jose J LopezJuan A Rosado
Jan 12, 2019·Journal of Thrombosis and Haemostasis : JTH·Indranil BasakPaul F Bray
Mar 6, 2019·Blood·Sjors G J G In 't Veld, Thomas Wurdinger
Jan 19, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Patrick Provost
Mar 23, 2018·Frontiers in Cardiovascular Medicine·Sophia Lazar, Lawrence E Goldfinger
Nov 16, 2018·Nature Reviews. Cardiology·Paola E J van der Meijden, Johan W M Heemskerk
Oct 24, 2018·Research and Practice in Thrombosis and Haemostasis·Marlie H Fisher, Jorge Di Paola
Apr 23, 2019·Wiley Interdisciplinary Reviews. RNA·MinJung KimTami J Kingsbury
Aug 4, 2019·International Journal of Molecular Sciences·Eun Ju KoNam Keun Kim
Jun 9, 2020·Molecular Biology Reports·Miruna-Mihaela MicheuIon-Andrei Nita
Oct 26, 2018·Platelets·Sanjeev RaghuwanshiRavi Kumar Gutti
Sep 7, 2018·Clinical Science·Voahanginirina Randriamboavonjy, Ingrid Fleming
Oct 18, 2019·Cells·Jersey Heitor da Silva MauésRommel Mário Rodriguez Burbano
Aug 19, 2020·Biomolecules·Jersey Heitor da Silva MauésRommel Mário Rodriguez Burbano
Feb 6, 2020·International Journal of Molecular Sciences·Bernadett SzilágyiBéla Nagy
Jan 21, 2017·Frontiers in Cell and Developmental Biology·Claire K S MeikleRandall G Worth
Mar 30, 2017·BioMed Research International·Ananília Medeiros Gomes da SilvaVivian Nogueira Silbiger
Sep 19, 2020·Journal of Thrombosis and Haemostasis : JTH·Frederik DenormeRobert A Campbell
Oct 21, 2020·International Journal of Molecular Sciences·Carolin T NeuMonika Haemmerle
Oct 31, 2020·Thrombosis and Haemostasis·Alix GarciaPierre Fontana
Dec 29, 2020·Seminars in Thrombosis and Hemostasis·Elisa DaneseGiuseppe Lippi
Nov 7, 2020·International Journal of Molecular Sciences·Anastasia KyselovaVoahanginirina Randriamboavonjy
Oct 15, 2020·Transfusion Medicine Reviews·Amal MaouiaJohn W Semple
Jun 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qun ZhangYong Song

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Nature Structural & Molecular Biology
Patricia LandryPatrick Provost
BMC Molecular Biology
Grace S TanMarianthi Kiriakidou
Advances in Experimental Medicine and Biology
Thomas C Roberts
Thrombosis and Haemostasis
T TreiberG Meister
© 2022 Meta ULC. All rights reserved