Dichroic components of Arsenazo III and dichlorophosphonazo III signals in skeletal muscle fibres

The Journal of Physiology
S M BaylorM W Marshall

Abstract

1. Absorbance changes were measured following stimulation of single muscle fibres injected with the metallochromic indicator dye Arsenazo III. Two dye-related signals can be clearly resolved: (1) an early, transient isotropic signal that appears to be due to the formation of Ca(2+):dye complex and (2) a slower, transient signal that is ;dichroic' in nature. The dichroic signal is obtained by taking the difference between absorbance changes measured with light plane polarized along the fibre axis (0 degrees light) and at right angles to the axis (90 degrees light).2. The time course of the dichroic signal is the same at all wavelengths employed, suggesting that a single underlying process is involved. The wavelength dependence of the magnitude of the signal is similar to that obtained for dye absorbance in a resting fibre.3. At 570 nm, near the isosbestic wavelength for changes in H(+):dye, Mg(2+):dye and Ca(2+):dye, the dichroic signal is near maximal. The absorbance change with 0 degrees light is positive and is about twice as large as the change with 90 degrees light, which is negative. This finding is consistent with the idea that the dichroic signal arises from dye molecules which change their orientation in the radially sy...Continue Reading

Citations

Mar 1, 1984·Cellular and Molecular Neurobiology·J A Connor, Z Ahmed
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·P PaladeG Hals
Jul 6, 2010·Progress in Biophysics and Molecular Biology·Stephen M Baylor, Stephen Hollingworth
Apr 1, 1985·Cell Calcium·J Vergara, M Delay
Aug 1, 1985·Journal of Muscle Research and Cell Motility·R F RakowskiM R James-Kracke
Feb 1, 1986·The American Journal of Physiology·P M Best, M Fill
Jan 1, 1982·Progress in Biophysics and Molecular Biology·J R BlinksF G Prendergast

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