Dictyostelium discoideum contains a family of calmodulin-related EF-hand proteins that are developmentally regulated

FEBS Letters
B AndréM Schleicher

Abstract

A full-length genomic DNA fragment that codes for a novel EF-hand protein Dictyostelium discoideum was cloned and sequenced. The protein is composed of 168 amino acids and contains four consensus sequences that are typical for (Ca2+)-binding EF-hand domains. The protein sequence exhibits only minor similarities to other calmodulin-type proteins from Dictyostelium. The genomic DNA harbors two short introns; their positions suggest that the gene is unrelated to the EF-hand proteins from the calmodulin group. Northern blot analysis showed that the mRNA level was significantly increased during development. Polyclonal antibodies raised against the recombinant protein recognized in Western blots a protein of about 20 kDa. Like the mRNA, also the protein was more abundant in developing cells. Overlay experiments with 45Ca2+ indicated that the EF-hands in fact have (Ca2+)-binding activity. The recent description of CBP1, another calmodulin-type Dictyostelium protein that is upregulated during development [Coukell et al. (1995) FEBS Lett. 362, 342-346], suggests that D. discoideum contains a family of EF-hand proteins that have specific functions during distinct steps of development. We therefore designate the protein described in this ...Continue Reading

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Citations

Sep 13, 2001·Molecular Biology of the Cell·N IranfarW F Loomis
Dec 21, 2004·Development, Growth & Differentiation·Barrie CoukellKatharine Shim
Apr 22, 2005·Biochimica Et Biophysica Acta·Chang-Hun LeeSa-Ouk Kang
Jul 19, 2017·The Journal of Eukaryotic Microbiology·Helmut Plattner
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Aug 25, 2004·Biochemical and Biophysical Research Communications·Michael A Myre, Danton H O'Day
Mar 17, 2005·Biochimica Et Biophysica Acta·Tsogbadrakh Mishig-OchiriinSa-Ouk Kang
Jun 10, 2006·European Journal of Cell Biology·Marija MarkoAngelika A Noegel
Sep 28, 2007·Cellular Signalling·Andrew Catalano, Danton H O'Day

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