PMID: 8609164Apr 1, 1996Paper

Dictyostelium mutants lacking multiple classic myosin I isoforms reveal combinations of shared and distinct functions

The Journal of Cell Biology
G JungJ A Hammer

Abstract

Dictyostelium cells that lack the myoB isoform were previously shown to exhibit reduced efficiencies of phagocytosis and chemotactic aggregation ("streaming") and to crawl at about half the speed of wild-type cells. Of the four other Dictyostelium myosin I isoforms identified to date, myoC and myoD are the most similar to myoB in terms of tail domain sequence. Furthermore, we show here that myoC, like myoB and myoD, is concentrated in actin-rich cortical regions like the leading edge of migrating cells. To look for evidence of functional overlap between these isoforms, we analyzed myoB, myoC, and myoD single mutants, myoB/myoD double mutants, and myoB/myoC/myoD triple mutants, which were created using a combination of gene targeting techniques and constitutive expression of antisense RNA. With regard to the speed of locomoting, aggregation-stage cells, of the three single mutants, only the myoB mutant was significantly slower. Moreover, double and triple mutants were only slightly slower than the myoB single mutant. Consistent with this, the protein level of myoB alone rises dramatically during early development, suggesting that a special demand is placed on this one isoform when cells become highly motile. We also found, howev...Continue Reading

References

Dec 1, 1992·The Journal of Cell Biology·I C BainesE D Korn
Jan 1, 1991·Cell Motility and the Cytoskeleton·D WesselsD R Soll
Feb 1, 1991·Current Opinion in Cell Biology·P A Janmey
Aug 14, 1986·Biochemical and Biophysical Research Communications·G Klein, M Satre
Nov 1, 1989·Cell Regulation·M A TitusJ A Spudich
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G JungJ A Hammer
Jan 1, 1988·Journal of Cellular Biochemistry·E D KornT J Lynch
Aug 1, 1994·The Journal of Cell Biology·C J CohenI Mellman
Oct 1, 1994·The Journal of Cell Biology·R A BaconI Mellman
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A TitusW F Loomis
Nov 1, 1994·The Journal of Cell Biology·P CupersP J Courtoy
Mar 1, 1994·The Journal of Cell Biology·L J HewlettC Watts
Nov 1, 1993·Trends in Genetics : TIG·J H Thomas
Dec 1, 1995·The Journal of Cell Biology·K D NovakM A Titus
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M S Mooseker, R E Cheney

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Citations

Jul 25, 1998·Cell Motility and the Cytoskeleton·X WuJ A Hammer
Nov 1, 2000·Cell Motility and the Cytoskeleton·N Osherov, G S May
Oct 17, 2002·The Anatomical Record·Reid F Thompson, George M Langford
Jul 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A M Gulick, I Rayment
Nov 27, 2002·Cell Motility and the Cytoskeleton·E Michael OstapThomas D Pollard
Dec 15, 1996·Experientia·H RiezmanL Hicke
Dec 15, 1996·Experientia·V R Simon, L A Pon
Jul 14, 1999·Cell Biochemistry and Biophysics·T SoldatiE C Schwarz
Jul 14, 1999·Cell Biochemistry and Biophysics·E C SchwarzT Soldati
Mar 21, 2001·Biochimica Et Biophysica Acta·M A de la Roche, G P Côté
Jan 16, 2002·Current Opinion in Immunology·Steven Greenberg, Sergio Grinstein
Feb 13, 2001·Current Opinion in Cell Biology·J Taunton
Mar 4, 1998·Current Opinion in Cell Biology·J P Baker, M A Titus
Feb 19, 2000·Current Opinion in Cell Biology·X WuJ A Hammer
Jul 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rajalakshmi NambiarMatthew J Tyska
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·H BrzeskaE D Korn
Mar 1, 2012·The Journal of Biological Chemistry·Hanna BrzeskaEdward D Korn
Nov 14, 2002·Molecular Biology of the Cell·Fatima-Zahra IdrissiM Isabel Geli
May 8, 1999·Molecular Biology of the Cell·G RaposoE Coudrier
Dec 12, 2001·Molecular Biology of the Cell·M N CordonnierE Coudrier
Mar 14, 1998·Molecular Biology of the Cell·K D Novak, M A Titus
Aug 7, 1998·Molecular Biology of the Cell·E L de HostosR D Vale
Oct 29, 2004·Molecular Biology of the Cell·Marc de la RocheGraham P Côté
Sep 15, 2006·Molecular Biology of the Cell·Margaret ClarkeGünther Gerisch
Sep 15, 2006·Molecular Biology of the Cell·David E HokansonE Michael Ostap
May 29, 2002·The EMBO Journal·Martin KollmarF Jon Kull
Feb 3, 2012·Science Signaling·Chun-Lin ChenMiho Iijima
Oct 8, 2004·Eukaryotic Cell·Ursula OberholzerMalcolm Whiteway
Jun 11, 2004·Annual Review of Biochemistry·Susanne M Rafelski, Julie A Theriot
Jan 19, 1999·Annual Review of Cell and Developmental Biology·P GunningE Hardeman
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Matthew D Welch, R Dyche Mullins
May 3, 2000·Cell Structure and Function·T Q UyedaS Yumura
Jun 2, 2010·HFSP Journal·Britta Schroth-DiezGünther Gerisch
Jan 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shi ShuEdward D Korn
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·David E Hokanson, E Michael Ostap

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