PMID: 2497773Mar 7, 1989Paper

Dicyclohexylcarbodiimide cross-links two conserved residues, Asp-184 and Lys-72, at the active site of the catalytic subunit of cAMP-dependent protein kinase

Biochemistry
J A Buechler, S S Taylor

Abstract

In the absence of MgATP, the catalytic subunit of cAMP-dependent protein kinase is irreversibly inhibited by the hydrophobic carbodiimide dicyclohexylcarbodiimide, and this inhibition is most likely due to the formation of a cross-link between a carboxyl group and a lysine residue in the active site (Toner-Webb & Taylor, 1987). In order to identify these cross-linked residues, the catalytic subunit was modified by dicyclohexylcarbodiimide and then treated with acetic anhydride and digested with trypsin. The resulting peptides were resolved by high-performance liquid chromatography. One major absorbing tryptic peptide and one smaller peptide consistently and reproducibly showed a decrease in absorbance after the catalytic subunit had been treated with DCCD. These peptides correspond to residues 166-190 and 57-93, respectively. A unique peptide was isolated from the modified catalytic subunit, and the sequence of this peptide established that the cross-linking occurred between Asp-184 and Lys-72. The cross-linking of these two residues, which were both identified previously as essential residues, confirms the likelihood that each plays a role in the functioning of this enzyme. The fact that Asp-184 and Lys-72 appear to be invaria...Continue Reading

References

Jul 1, 1987·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S S Taylor
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W C Barker, M O Dayhoff
Jan 1, 1984·CRC Critical Reviews in Biochemistry·H N BramsonA S Mildvan

❮ Previous
Next ❯

Citations

Jan 1, 1993·Molecular & General Genetics : MGG·S A MacNeill, P Nurse
Mar 25, 2004·Protein Expression and Purification·Weiqing ZhangStephen J Beebe
Nov 26, 2003·International Journal for Parasitology·Martin WieseIris Görcke
Aug 15, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H KatoH Kaneko
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·W LorenzR J Lefkowitz
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A C CarreraT M Roberts
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·K J CokerC A Guyer
Feb 10, 2006·Molecular Biology of the Cell·Maja ErdmannMartin Wiese
Feb 25, 1991·Nucleic Acids Research·E KhanA M Skalka
Mar 1, 1993·Trends in Biochemical Sciences·K Riehemann, C Sorg
May 16, 1990·Biochemical and Biophysical Research Communications·T Takahashi, K Takahashi
Mar 1, 1993·Trends in Biochemical Sciences·S S TaylorM J Zoller
Jun 29, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S S TaylorW M Yonemoto
May 7, 2019·IUBMB Life·Susan S TaylorAlexandr P Kornev
Jul 26, 1996·The Journal of Biological Chemistry·C R McCudden, S G Powers-Lee
Dec 25, 2004·The Journal of Biological Chemistry·Ganesh H IyerSusan S Taylor
Dec 19, 2019·The Journal of Organic Chemistry·Kate J McKnellyJames S Nowick

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.