Dielectrophoretic analysis of changes in cytoplasmic ion levels due to ion channel blocker action reveals underlying differences between drug-sensitive and multidrug-resistant leukaemic cells

Physics in Medicine and Biology
L DuncanF H Labeed

Abstract

Dielectrophoresis (DEP)--the motion of particles in non-uniform AC fields-has been used in the investigation of cell electrophysiology. The technique offers the advantages of rapid determination of the conductance and capacitance of membrane and cytoplasm. However, it is unable to directly determine the ionic strengths of individual cytoplasmic ions, which has potentially limited its application in assessing cell composition. In this paper, we demonstrate how dielectrophoresis can be used to investigate the cytoplasmic ion composition by using ion channel blocking agents. By blocking key ion transporters individually, it is possible to determine their overall contribution to the free ions in the cytoplasm. We use this technique to evaluate the relative contributions of chloride, potassium and calcium ions to the cytoplasmic conductivities of drug sensitive and resistant myelogenous leukaemic (K562) cells in order to determine the contributions of individual ion channel activity in mediating multi-drug resistance in cancer. Results indicate that whilst K(+) and Ca(2+) levels were extremely similar between sensitive and resistant lines, levels of Cl(-) were elevated by three times to that in the resistant line, implying increased...Continue Reading

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Citations

Oct 8, 2011·PloS One·Fatima H LabeedLisa A Flanagan
May 6, 2015·International Journal of Molecular Sciences·Jian ChenJunbo Wang
Jul 3, 2013·Biotechnology and Bioengineering·Katrin BraaschMichael Butler
May 23, 2015·Journal of the Royal Society, Interface·T TaghianA B Kogan
May 21, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Julia FredeMustafa B A Djamgoz
Dec 8, 2017·Nature Communications·Erin A HensleeFatima H Labeed
Feb 7, 2009·Electrophoresis·Michelle M MeighanMark A Hayes
Nov 5, 2015·Electrophoresis·Bashar YafouzFatimah Ibrahim
Dec 14, 2018·Scientific Reports·Azita FazelkhahDouglas Thomson
Apr 28, 2012·Physics in Medicine and Biology·C ChampionJ Hanssen
Apr 15, 2017·Proceedings of the National Academy of Sciences of the United States of America·Shabnam A FaraghatMichael P Hughes
Apr 29, 2021·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mei JiangWentao Yue

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