Dielectrophoretic capture of mammalian cells using transparent indium tin oxide electrodes in microfluidic systems

Electrophoresis
Bharat SankaranSamuel P Forry

Abstract

Transparent indium tin oxide microelectrodes were fabricated and used to immobilize suspended NIH 3T3 fibroblast cells by positive dielectrophoresis. The indium tin oxide electrodes facilitated microscopic observation of immobilized cells compared with opaque metallized electrodes. Dielectrophoresis was used to capture arrays of individual cells and form small cell clusters within a microfluidic network. The extent of cellular immobilization (no-cell, single-cell, or multiple-cell capture) was correlated with the applied voltage and inversely with the flow velocity. Specific conditions yielding predominantly single-cell capture were identified. The viability of immobilized cells was confirmed using fluorescence microscopy.

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Citations

Sep 17, 2011·Analytical and Bioanalytical Chemistry·Elisa Michelini, Aldo Roda
Sep 17, 2010·Analytical Chemistry·Jay WarrickDavid Beebe
Oct 15, 2011·Lab on a Chip·Samuel P Forry, Laurie E Locascio
Apr 4, 2012·Annual Review of Chemical and Biomolecular Engineering·Frederik S O FritzschAndreas Schmid
Sep 17, 2011·Electrophoresis·Barbaros Cetin, Dongqing Li
Dec 17, 2009·Electrophoresis·Roberto C Gallo-VillanuevaBlanca H Lapizco-Encinas
May 14, 2010·Analytical Chemistry·Georgette B Salieb-BeugelaarAndreas Manz
Aug 6, 2010·Analytical Chemistry·Fabrice GielenJoshua B Edel

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