Dielectrophoretic separation of colorectal cancer cells.

Biomicrofluidics
Fang YangGuiren Wang

Abstract

Separation of colorectal cancer cells from other biological materials is important for stool-based diagnosis of colorectal cancer. In this paper, we use conventional dielectrophoresis in a microfluidic chip to manipulate and isolate HCT116 colorectal cancer cells. It is noticed that at a particular alternating current frequency band, the HCT116 cells are clearly deflected to a side channel from the main channel after the electric activation of an electrode pair. This motion caused by negative dielectrophoresis can be used to simply and rapidly separate cancer cells from other cells. In this manuscript, we report the chip design, flow conditions, dielectrophoretic spectrum of the cancer cells, and the enrichment factor of the colorectal cancer cells from other cells.

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Citations

Oct 23, 2010·Analytical and Bioanalytical Chemistry·Soumya K SrivastavaAdrienne R Minerick
Mar 26, 2014·Cancers·Peter R C Gascoyne, Sangjo Shim
Aug 11, 2010·Biomicrofluidics·Miguel SanchoRonald Pethig
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Jan 10, 2014·Biomicrofluidics·Mohammed AlshareefGuiren Wang
Jul 21, 2010·Biomicrofluidics·Naga Siva Kumar Gunda, Sushanta Kumar Mitra
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Sep 17, 2011·Electrophoresis·Barbaros Cetin, Dongqing Li
Aug 9, 2011·Electrophoresis·Fang YangGuiren Wang
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May 9, 2021·Nature Methods·Luca FiorilloMario Nicodemi

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Methods Mentioned

BETA
electrophoresis
chip

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