Dielectrophoretic stretching of DNA tethered to a fiber tip

Nanotechnology
Changbae HyunJiali Li

Abstract

In this work, we studied the stretching of λ phage DNA molecules immobilized on an optical fiber tip attached to a force sensitive tuning fork under ac electric fields. We designed a two electrodes stretching system in a small chamber: one is a gold-coated optical fiber tip electrode, and the other is a gold-coated flat electrode. By applying a dielectrophoretic (DEP) force, the immobilized λ DNA molecules on the tip are stretched and the stretching process is monitored by a fluorescent microscope. The DNA stretching in three-dimensional space is optimized by varying electrode shape, electrode gap distance, ac frequency, and solution conductivity. By observing the vibrational amplitude change of a quartz tuning fork, we measured the effects due to Joule heating and the DEP force on the tethered DNA molecules in solution. This work demonstrates a method to manipulate and characterize immobilized λ DNA molecules on a probe tip for further study of single DNA molecules.

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Citations

May 10, 2017·ACS Applied Materials & Interfaces·Peilin ZhouLianqing Liu
Mar 2, 2017·Sensors·Nurhaslina Abd RahmanBashar Yafouz
Oct 3, 2020·The Review of Scientific Instruments·Shaoxi FangDeqiang Wang
Sep 6, 2019·Sensors·Fernando-Juan García-DiegoFrancisco J Espinós
Dec 8, 2019·Sensors·García-Diego Fernando-JuanFrancisco J Espinós
Mar 3, 2017·The Review of Scientific Instruments·Changbae HyunJiali Li
Aug 14, 2020·ACS Combinatorial Science·Ina Turcan, Marius Andrei Olariu

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