Dienogest reduces HSD17β1 expression and activity in endometriosis

The Journal of Endocrinology
Taisuke MoriJ Kitawaki

Abstract

Endometriosis is an estrogen-dependent disease. Abnormally biosynthesized estrogens in endometriotic tissues induce the growth of the lesion and worsen endometriosis-associated pelvic pain. Dienogest (DNG), a selective progesterone receptor agonist, is widely used to treat endometriosis and efficiently relieves the symptoms. However, its pharmacological action remains unknown. In this study, we elucidated the effect of DNG on enzymes involved in local estrogen metabolism in endometriosis. Surgically obtained specimens of 23 ovarian endometriomas (OE) and their homologous endometrium (EE), ten OE treated with DNG (OE w/D), and 19 normal endometria without endometriosis (NE) were analyzed. Spheroid cultures of stromal cells (SCs) were treated with DNG and progesterone. The expression of aromatase, 17β-hydroxysteroid dehydrogenase 1 (HSD17β1), HSD17β2, HSD17β7, HSD17β12, steroid sulfatase (STS), and estrogen sulfotransferase (EST) was evaluated by real-time quantitative PCR. The activity and protein level of HSD17β1 were measured with an enzyme assay using radiolabeled estrogens and immunohistochemistry respectively. OESCs showed increased expression of aromatase, HSD17β1, STS, and EST, along with decreased HSD17β2 expression, whe...Continue Reading

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Citations

Jan 9, 2016·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Antonio Simone LaganàVittorio Italo Palmara
Dec 10, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Giovanni GrandiBrett McKinnon
Jan 3, 2016·The Journal of Steroid Biochemistry and Molecular Biology·Carla A PiccinatoRui A Ferriani
Feb 25, 2017·Acta Obstetricia Et Gynecologica Scandinavica·Erin GreavesPhilippa T K Saunders
Nov 23, 2017·Reproductive Sciences·Sa Ra LeeSung Hoon Kim
Jul 26, 2018·The Journal of Obstetrics and Gynaecology Research·Taisuke MoriJo Kitawaki
Feb 16, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·V TskhayG Мikailly
Sep 29, 2019·Journal of Cellular and Molecular Medicine·Wenqian XiongYi Liu
Jul 15, 2020·Hormone Molecular Biology and Clinical Investigation·Michael OettelKlaus Nickisch
Apr 14, 2017·Journal of Assisted Reproduction and Genetics·Maciej OsińskiMalgorzata Szczepańska
Dec 3, 2016·Archives of Gynecology and Obstetrics·Antonio Simone LaganàAndrea Romano
Oct 15, 2019·Reproductive Medicine and Biology·Taisuke MoriJo Kitawaki
Jun 30, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Samira AsghariMehdi Yousefi

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