PMID: 8597582Feb 16, 1996Paper

Diet fat alters expression of genes for enzymes of lipogenesis in lean and obese mice

Biochimica Et Biophysica Acta
S K Cheema, M T Clandinin

Abstract

The objective of this experiment was to determine the effect of polyunsaturated fatty acids on gene expression for fatty acid synthase, acetyl CoA-carboxylase, malic enzyme, pyruvate kinase, and phosphoenolpyruvate carboxykinase in obese mice. Eight-week-old female lean and obese mice were fed semi-purified diets containing 20% (w/w) fat of either high or low polyunsaturated to saturated (P/S) fatty acid ratio for four weeks. Total RNA was isolated from liver and was hybridized to cDNA probes for the above enzymes. Consumption of a high P/S diet decreased mRNA levels for all the lipogenic enzymes studied in both lean and obese mice. Compared to lean mice, obese mice exhibited a higher mRNA level for fatty acid synthase, acetyl CoA-carboxylase, malic enzyme, and pyruvate kinase in animals fed either a high or low P/S diet. Enzyme-specific activities followed the same profile as the mRNA levels in both lean and obese mice fed a high or low P/S diet. The decrease in liver fatty acid synthase mRNA level was more pronounced in lean mice compared to obese mice, suggesting that the obese mice may be more resistant to polyunsaturated fatty acid feedback control of gene expression.

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Citations

Oct 8, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·J SalehK Cianflone
Nov 10, 2005·Public Health Nutrition·Ricardo Uauy, Erik Díaz
Jan 1, 1997·Annual Review of Nutrition·K H Kim
Nov 5, 1997·Annals of the New York Academy of Sciences·N A Gletsu, M T Clandinin
Nov 5, 1997·Annals of the New York Academy of Sciences·E Seböková, I Klimes
Aug 25, 1999·Metabolism: Clinical and Experimental·Y ZhangA H Cincotta

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