Diet-induced obesity in young mice: Consequences on the pancreatic intrinsic nervous system control of insulin secretion

Endocrinology, Diabetes & Metabolism
Marie-Béatrice SaadeSophie Blat

Abstract

Obesity has become a pandaemic even in children. We aimed to investigate the impact of obesity in youth on later pancreatic intrinsic nervous system (PINS) phenotype and control of insulin secretion. Young mice (5-week-old, T0 group) were fed either a normal diet (ND group) or a Western diet (WD group) for 12 weeks. Pancreas nervous system density, PINS phenotype and pancreas anatomy were analysed by immunohistochemistry at T0 and in adulthood (ND and WD groups). Insulin secretion was also studied in these 3 groups using a new model of ex vivo pancreatic culture, where PINS was stimulated by nicotinic and nitrergic agonists with and without antagonists. Insulin was assayed in supernatants by ELISA. Pancreas nervous system density decreased with age in ND (P < .01) but not in WD mice (P = .08). Western diet decreased the PINS nitrergic component as compared to normal diet (P < .01) but it did not modify its cholinergic component (P = .50). Nicotinic PINS stimulation induced greater insulin secretion in ND compared to WD mice (P < .001) whereas nitrergic stimulation significantly decreased insulin secretion in ND mice (P < .001) and tended to increase insulin secretion in WD mice (P = .08). Endocrine pancreas anatomy was not modi...Continue Reading

References

Jun 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A L KirchgessnerM D Gershon
Jun 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H L Kaung
Jan 22, 1998·The American Journal of Physiology·M T Liu, A L Kirchgessner
Apr 21, 1998·European Journal of Pharmacology·S Karlsson, B Ahrén
Jan 15, 1999·Pancreas·B AhrénF Sundler
Jul 17, 1999·The American Journal of Physiology·K L Teff, R R Townsend
Jan 20, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N WierupF Sundler
Feb 13, 2007·Journal of Molecular Endocrinology·Amanda M Ackermann, Maureen Gannon
Jun 26, 2007·Diabetes, Obesity & Metabolism·N R HillD R Matthews
Jun 30, 2009·Academic Pediatrics·Joseph A SkeltonSarah E Barlow
Jul 4, 2012·Free Radical Biology & Medicine·Thomas NyströmÅke Sjöholm
Sep 10, 2014·Diabetes, Obesity & Metabolism·S-C TangH-J Chien
Sep 10, 2014·Diabetes, Obesity & Metabolism·E Gylfe, A Tengholm
Feb 1, 2015·Molecular Aspects of Medicine·Leslie S SatinArthur S Sherman
Jul 29, 2015·Diabetologia·Rafael Arrojo e DrigoBernhard O Boehm
Aug 31, 2017·Diabetologia·Shiue-Cheng TangMichael S German
Sep 3, 2017·Diabetologia·Shiue-Cheng TangPankaj J Pasricha

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