Dietary Bacillus subtilis-based direct-fed microbials alleviate LPS-induced intestinal immunological stress and improve intestinal barrier gene expression in commercial broiler chickens.

Research in Veterinary Science
Ujvala Deepthi GaddeHyun S Lillehoj

Abstract

This study investigated the effects of Bacillus subtilis-based probiotics on the performance, modulation of host inflammatory responses and intestinal barrier gene expression of broilers subjected to LPS challenge. Chickens were randomly allocated to one of the 3 dietary treatment groups - control, antibiotic, or probiotic. At 14days, half of the chickens in each treatment were injected with LPS (1mg/kg body weight), and the other half injected with sterile PBS. Chickens fed probiotics weighed significantly more than controls at 15days of age, irrespective of immune challenge. LPS challenge significantly reduced weight gain at 24h post-injection, and the probiotics did not alleviate the LPS-induced reduction of weight gain. Serum α-1-AGP levels were significantly higher in LPS-injected chickens, and probiotic supplementation significantly reduced their levels. The percentages of CD4+ lymphocytes were significantly increased in probiotic groups in the absence of immunological challenge but were reduced during LPS challenge compared to controls. CD8+ lymphocytes were significantly reduced in probiotic-fed birds. The LPS-induced increase in the expression of cytokines IL8 and TNFSF15 was reduced by probiotic supplementation, and I...Continue Reading

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Citations

Apr 11, 2018·Avian Pathology : Journal of the W.V.P.A·Ar'Quette GrantHyun S Lillehoj
Feb 20, 2019·Experimental Biology and Medicine·Se Jin SongRob Knight
Dec 10, 2019·Journal of Applied Microbiology·H A G DucrayI Sorokulova
Apr 26, 2020·Applied and Environmental Microbiology·Samiullah KhanKapil K Chousalkar
Apr 25, 2020·Frontiers in Veterinary Science·Ibrahim F RehanAbd El-Latif Hesham
Apr 11, 2021·Applied and Environmental Microbiology·A EregaI Mandic Mulec
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Igor V PopovMichael L Chikindas
Aug 17, 2021·Trends in Microbiology·Kasun H BodawattaKnud A Jønsson

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