Dietary calcium regulates ROS production in aP2-agouti transgenic mice on high-fat/high-sucrose diets

International Journal of Obesity : Journal of the International Association for the Study of Obesity
X Sun, M B Zemel

Abstract

We have previously demonstrated that 1alpha, 25(OH)2D3 promotes adipocyte reactive oxygen species (ROS) production. We have now evaluated whether decreasing 1alpha, 25(OH)2D3 levels by increasing dietary calcium will decrease oxidative stress in vivo. We fed low-calcium (0.4% Ca) and high-calcium (1.2% Ca from CaCO3) obesity-promoting (high sucrose/high fat) diets to aP2-agouti transgenic mice and assessed regulation of ROS production in adipose tissue and skeletal muscle. Mice on the high-calcium diet gained 50% of the body weight (P=0.04) and fat (P<0.001) as mice on the low-calcium diet (0.4% Ca). The high-calcium diet significantly reduced adipose intracellular ROS production by 64 and 18% (P<0.001) and inhibited adipose tissue nicotinamide adenine dinucleotide phosphate oxidase expression by 49% (P=0.012) and 63% (P=0.05) in visceral and subcutaneous adipose tissue, respectively. Adipocyte intracellular calcium ([Ca2+]i) levels were suppressed in mice on the high-calcium diet by 73-80% (P<0.001). The high-calcium diet also induced 367 and 191% increases in adipose mitochondrial uncoupling protein 2 (UCP2) expression (P<0.001) in visceral and subcutaneous adipose tissue, respectively. The pattern of UCP3 expression and indi...Continue Reading

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Citations

Aug 19, 2007·The British Journal of Nutrition·Taru K PilviEero M Mervaala
Jul 1, 2011·The American Journal of Clinical Nutrition·Renée A StancliffeMichael B Zemel
Dec 5, 2008·Nutrition Reviews·Michael B Zemel, Xiaocun Sun
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Aug 30, 2011·Metabolism: Clinical and Experimental·Rihua ZhangYun Liu
Sep 18, 2007·The Journal of Nutritional Biochemistry·Xiaocun Sun, Michael B Zemel
Jun 26, 2007·The Journal of Nutritional Biochemistry·Pilar ParraFrancisca Serra
Feb 15, 2007·Obesity·Xiaocun Sun, Michael B Zemel
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Nov 6, 2009·The American Journal of Clinical Nutrition·Michael B ZemelBeth Wilson
Jan 6, 2010·The Physician and Sportsmedicine·Michael B Zemel
May 22, 2008·The Journal of Nutrition·Michael B Zemel, Xiaocun Sun
Apr 4, 2021·Nutrients·Mateusz Jakub KarniaJan Jacek Kaczor

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