Dietary Fiber Treatment Corrects the Composition of Gut Microbiota, Promotes SCFA Production, and Suppresses Colon Carcinogenesis

Genes
Faraz BishehsariAli Keshavarzian

Abstract

Epidemiological studies propose a protective role for dietary fiber in colon cancer (CRC). One possible mechanism of fiber is its fermentation property in the gut and ability to change microbiota composition and function. Here, we investigate the role of a dietary fiber mixture in polyposis and elucidate potential mechanisms using TS4Cre×cAPCl°x468 mice. Stool microbiota profiling was performed, while functional prediction was done using PICRUSt. Stool short-chain fatty acid (SCFA) metabolites were measured. Histone acetylation and expression of SCFA butyrate receptor were assessed. We found that SCFA-producing bacteria were lower in the polyposis mice, suggesting a decline in the fermentation product of dietary fibers with polyposis. Next, a high fiber diet was given to polyposis mice, which significantly increased SCFA-producing bacteria as well as SCFA levels. This was associated with an increase in SCFA butyrate receptor and a significant decrease in polyposis. In conclusion, we found polyposis to be associated with dysbiotic microbiota characterized by a decline in SCFA-producing bacteria, which was targetable by high fiber treatment, leading to an increase in SCFA levels and amelioration of polyposis. The prebiotic activi...Continue Reading

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Dec 23, 1999·The Journal of Biological Chemistry·J R Saam, J I Gordon
Jan 5, 2002·Journal of Nutritional Science and Vitaminology·T Sugawara, T Miyazawa
May 13, 2003·The Proceedings of the Nutrition Society·Sandra Macfarlane, George T Macfarlane
Jul 4, 2003·The Journal of Nutrition·James R Davie
Aug 9, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Denis E Corpet, Fabrice Pierre
Aug 25, 2006·Molecular Carcinogenesis·Volker MaiStephen D Hursting
Oct 16, 2007·Clinical Microbiology Reviews·Hannah M Wexler
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Elias GounarisKhashayarsha Khazaie
Dec 26, 2008·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Henrike M HamerRobert-Jan M Brummer
Sep 11, 2009·The Journal of Nutrition·Stephen J D O'KeefeThomas Mawhinney
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Aug 17, 2010·Bioinformatics·Robert C Edgar
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Feb 8, 2011·PloS One·Iradj SobhaniJean P Furet
Jan 1, 2008·CSH Protocols·Andrew H FischerRolf Zeller
Mar 15, 2011·FEMS Microbiology Ecology·Alvaro BelenguerGerald E Lobley
Apr 8, 2011·World Journal of Gastroenterology : WJG·Roberto Berni CananiAntonio Calignano
Sep 15, 2011·Biochimica Et Biophysica Acta·Melissa J PuppaJames A Carson
Jan 19, 2012·Nutrients·Asa Hakansson, Goran Molin
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Khashayarsha KhazaieMansour Mohamadzadeh
Jun 16, 2012·Nature·UNKNOWN Human Microbiome Project Consortium
Jul 17, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Alda J Leonel, Jacqueline I Alvarez-Leite
Feb 12, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qingchao ZhuHuanlong Qin
Feb 16, 2013·Medical Hypotheses·Hassan Tirandaz, Ebrahim Mohammadi
May 2, 2013·Journal of Proteome Research·Ying ZhaoYulan Wang
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower
Aug 28, 2013·Current Opinion in Pharmacology·Vadivel GanapathyNagendra Singh
Nov 7, 2013·MBio·Joseph P ZackularPatrick D Schloss
Feb 28, 2014·World Journal of Gastroenterology : WJG·Georgina L HoldIndrani Mukhopadhya
May 28, 2014·FEMS Microbiology Reviews·Mirjana Rajilić-Stojanović, Willem M de Vos
Aug 5, 2014·Journal of Molecular Biology·Bruce R Hamaker, Yunus E Tuncil

❮ Previous
Next ❯

Citations

Jul 28, 2018·Molecular Nutrition & Food Research·Miriam G ShtrikerZecharia Madar
Jun 4, 2020·Cancers·Lidia Sánchez-AlcoholadoMaría Isabel Queipo-Ortuño
Mar 25, 2019·Frontiers in Immunology·Pushpa PandiyanJochen Huehn
Feb 2, 2019·Experimental Biology and Medicine·Megan T Zangara, Christine McDonald
Aug 28, 2020·Frontiers in Oncology·Faraz BishehsariYue Zhang
Dec 12, 2018·European Journal of Clinical Nutrition·Yutong DongHaidong Zhu
Aug 12, 2018·Nutrients·Tiehua ZhangChanghui Zhao
Jul 19, 2019·Nutrients·Ronald D HillsCory R Theberge
Dec 14, 2018·Nutrients·Karolina Skonieczna-ŻydeckaEwa Stachowska
Jun 24, 2018·European Journal of Pharmacology·Marco RossiFaraz Bishehsari
Oct 20, 2020·Microbial Pathogenesis·Amir Hossein MohseniXiangsheng Fu
Apr 4, 2021·International Journal of Molecular Sciences·Suzanne AbbringBetty C A M van Esch
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mudhi AlAliSwee-Hua Erin Lim
Apr 18, 2021·International Journal of Biological Macromolecules·Pedro J Fernandez-JuliaDouwe van Sinderen
May 8, 2021·Frontiers in Pharmacology·Lingli RenYang Yang
May 22, 2021·Critical Reviews in Food Science and Nutrition·Riya RajeevJoseph Selvin
Jun 4, 2021·Journal of Agricultural and Food Chemistry·Aishwarya Naik, Santosh K Misra
Jun 5, 2021·Critical Reviews in Food Science and Nutrition·Junjie LinChun Cui
Jul 3, 2021·Microorganisms·Manijeh MahdaviEric Massé
Jul 16, 2021·Therapeutic Advances in Infectious Disease·Kristine M ErlandsonCara C Wilson
Jul 27, 2021·British Poultry Science·Shuang LiuHai Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay
electrophoresis
histone acetylation

Software Mentioned

uclust
Primer7
NIH Image J
PEAR (
PICRUSt
vegan
QIIME
SAS
PICRUSt ( Phylogenetic Investigation of Communities by Reconst...
CLC Genomics Workbench

Related Concepts

Related Feeds

Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.