Dietary glycosaminoglycans interfere in bacterial adhesion and gliadin-induced pro-inflammatory response in intestinal epithelial (Caco-2) cells

International Journal of Biological Macromolecules
J M LaparraY Sanz

Abstract

Dietary components may have an important role in maintaining a balanced gut microbiota composition. Celiac disease is an autoimmune enteropathy caused by gliadins, and has been associated with a reduced proportion of Bifidobacterium in gut microbiota. This study evaluates the influence of glycosaminoglycans (GAGs) on bacterial adhesion and their contribution in the gliadins-induced inflammatory response. The adhesion of potential probiotic (Bifidobacterium longum CECT 7347 and Bifidobacterium bifidum CECT 7365), commensal (Escherichia coli and Bacteroides fragilis) and pathogenic (Salmonella enterica CECT 443 and Listeria monocytogenes CECT 935) bacteria to mucin and Caco-2 cell cultures was determined. Gliadins were subjected to in vitro digestion (pepsin/pancreatin-bile), with/out GAGs, and the presence or not of cell suspensions of B. longum (10(8) CFU/ml). B. longum, E. coli, and L. monocytogenes, markedly interact with the high-sulphur-containing fraction of GAGs. The GAGs reduced the gliadins-mediated production of interleukin-1β, but not tumour necrosis factor-α. The results suggest that GAGs may ameliorate gliadin-induced inflammatory response, though they also slightly interfere with the action of B. longum.

References

Jul 1, 1991·Trends in Pharmacological Sciences·K A Karlsson
Oct 16, 1999·Science·G VenkataramanR Sasisekharan
Feb 7, 2001·European Journal of Gastroenterology & Hepatology·C G BeckettP J Ciclitira
May 14, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Maria Chiara MaiuriRosa Carnuccio
Sep 26, 2003·Best Practice & Research. Clinical Gastroenterology·Alain L Servin, Marie-Hélène Coconnier
Feb 8, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Monica G TingbøKirsten O Hannesson
Nov 3, 2005·Nature Clinical Practice. Gastroenterology & Hepatology·Ludvig M Sollid, Chaitan Khosla
Feb 14, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dongmei YeThomas Y Ma
May 20, 2006·Biochemical Society Transactions·E A YatesD G Fernig
Feb 3, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kirsten O HannessonRagni Ofstad
Mar 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rana M Al-Sadi, Thomas Y Ma
Apr 24, 2007·The Journal of Nutrition·Stefan Storcksdieck genannt BonsmannRichard F Hurrell
Sep 25, 2007·FEMS Microbiology Letters·Mónica Perea VélezJos Vanderleyden
Mar 5, 2008·Alimentary Pharmacology & Therapeutics·R TronconeUNKNOWN Members of European Multistakeholder Platform on CD (CDEUSSA)
Jun 10, 2008·Applied and Environmental Microbiology·Simone GuglielmettiCarlo Parini
Sep 24, 2008·Current Opinion in Structural Biology·Anne Imberty, Annabelle Varrot
Oct 15, 2008·Journal of Agricultural and Food Chemistry·José Moisés LaparraDennis D Miller
Nov 11, 2008·Journal of Clinical Pathology·M C ColladoY Sanz
Nov 15, 2008·Journal of Cellular Biochemistry·Giuseppe M CampoAlberto Calatroni
Feb 17, 2009·The Journal of General Virology·Laurent GilletPhilip G Stevenson
Mar 12, 2009·Mini Reviews in Medicinal Chemistry·Yolanda Sanz
May 20, 2009·Current Microbiology·Simone GuglielmettiDiego Mora
Jun 25, 2009·Analytical Chemistry·Bérangère TissotStuart M Haslam
Oct 22, 2009·Letters in Applied Microbiology·J M Laparra, Y Sanz

❮ Previous
Next ❯

Citations

Aug 3, 2013·The Journal of Allergy and Clinical Immunology·Renee L GreerNatalia Shulzhenko

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.