Dietary, nondigestible oligosaccharides and Bifidobacterium breve M-16V suppress allergic inflammation in intestine via targeting dendritic cell maturation

Journal of Leukocyte Biology
Sander de KivitLinette E M Willemsen

Abstract

Dietary intervention with short-chain galacto-oligosaccharides (scGOS), long-chain fructo-oligosaccharides (lcFOS) and Bifidobacterium breve M-16V (Bb) (GF/Bb) suppresses food allergic symptoms in mice, potentially via intestinal epithelial cell (IEC)-derived galectin-9. Furthermore, in vitro studies showed galacto- and fructo-oligosaccharides (GF) to enhance the immunomodulatory capacity of a TLR9 ligand representing bacterial CpG DNA when exposed to IEC. In this study, we investigated whether GF/Bb modulates dendritic cells (DCs) and subsequent Th2 and regulatory T cell (Treg) frequency in the small intestinal lamina propria (SI-LP). BALB/c mice were fed GF/Bb during oral OVA sensitization. DC and T cell phenotype were determined in SI-LP mononuclear cells using flow cytometry. Murine bone marrow-derived DCs (BMDCs) were exposed to recombinant galectin-9 or human monocyte-derived DCs (moDCs) and were cultured in IEC-conditioned medium from GF and TLR9 ligand-exposed HT-29 cells. GF/Bb reduced allergic symptoms and enhanced serum galectin-9 levels, while suppressing activation, restoring phagocytic capacity, and normalizing CD103 expression of SI-LP DCs of OVA-allergic mice. In vitro, galectin-9 suppressed LPS-induced activati...Continue Reading

Citations

Aug 18, 2017·Nutrients·Daniel MunblitJohn O Warner
Jan 12, 2019·Food & Function·R MukherjeeL E M Willemsen
Mar 5, 2019·Beneficial Microbes·V B M PetersM Meijerink
Apr 25, 2019·BMC Bioinformatics·Jolanda H M van BilsenTim J van den Broek
Oct 9, 2019·Allergy·Thomas EiweggerJulia Upton
Aug 28, 2020·Frontiers in Pediatrics·Giuliana FerranteMario Giuffrè

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