Dietary polysaccharide extracts of Agaricus brasiliensis fruiting bodies: chemical characterization and bioactivities at different levels of purification

Food Research International
Maja KozarskiLeo J L D Van Griensven

Abstract

Polysaccharides of the European strain of A. brasiliensis were obtained by hot water extraction and ethanol precipitation (HWPE I) of fruiting bodies, and further purified by dialysis (HWPE II) and pronase incubation (PPE). These polysaccharides consisted mainly of (1→6)-β-d-glucans. PPE was free of proteins and polyphenols as demonstrated by quantitative assays and NMR profiling. They showed a clear IFN-γ inducing activity in human PBMCs, which suggests these polysaccharides to have proinflammatory effects. Treatment by β-glucosidase caused the polysaccharides to be degraded into smaller fragments and at the same time increased their IFN-γ inducing activity in PBMCs fourfold. In vitro, PPE showed a dose-dependent inhibition of the proliferation of the human leukemia Jurkat cell. At 100μg/mL the cells' viability was decreased by appr. 51% compared to the control. EPR spin trapping demonstrated a high antioxidative activity against •OH and •O2- radicals of HWPE I and PPE. Further, the results of the antioxidant assays indicated that antioxidant activity against •OH radicals in the Fenton system was achieved through scavenging or through chelating iron mechanisms. The good immunomodulating and antioxidative properties of A. brasi...Continue Reading

Citations

Oct 31, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maja KozarskiLeo van Griensven
Apr 18, 2018·Applied Biochemistry and Biotechnology·Fengxia WangJi Zhang
May 18, 2019·Plant Foods for Human Nutrition·Fariba Ghiamati YazdiGert Folkerts
Feb 10, 2018·Anais Da Academia Brasileira De Ciências·Jevrosima StevanovicLeo J L D VAN Griensven
Jan 21, 2017·Current Pharmaceutical Design·Aline Cristine da Silva de SouzaRosane Marina Peralta

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