Dietary protein composition influences abundance of peptide and amino acid transporter messenger ribonucleic acid in the small intestine of 2 lines of broiler chicks

Poultry Science
Elizabeth R GilbertE A Wong

Abstract

This study evaluated the effect of dietary protein composition on mRNA abundance of a peptide transporter (peptide transporter 1, PepT1), amino acid (AA) transporters [Na(+)-independent cationic and zwitterionic AA transporter (b(o,+)AT), excitatory AA transporter 3 (EAAT3), Na(+)-independent cationic and Na(+)-dependent neutral AA transporter 2 (y(+)LAT2), L-type AA transporter 1 (LAT1), and cationic AA transporter 1 (CAT1)], and a digestive enzyme (aminopeptidase N) in 2 lines (A and B) of broilers that differentially express PepT1 mRNA (line B > line A). From d 8 to 15 posthatch, birds were fed 1 of 3 diets. Protein sources included whey protein concentrate, a whey partial hydrolysate (WPH), or a mixture of free AA (AA) identical to the composition of whey. Quantities of mRNA were assayed by real-time PCR in the small intestine of males at d 8, 9, 11, 13, and 15. For all genes except LAT1, abundance of mRNA was greatest in line B birds that consumed the WPH diet (P < 0.006). When mRNA abundance was normalized to beta-actin quantities, this effect disappeared, demonstrating a generalized effect on gene expression in line B birds that consumed the hydrolysate. There was a greater villus height:crypt depth ratio (P < 0.05) in l...Continue Reading

Citations

Aug 10, 2016·Probiotics and Antimicrobial Proteins·Ivelina PavlovaJohanna Fink-Gremmels
Jul 28, 2016·International Journal of Food Sciences and Nutrition·Mengjie LiGuanghong Zhou
Nov 3, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Tiziano VerriAlessandro Romano
Aug 29, 2018·Journal of Animal Physiology and Animal Nutrition·Reza MahdaviGleb Yurievich Kosovsky
Sep 5, 2019·Journal of Animal Physiology and Animal Nutrition·Boru ZhangAnshan Shan
May 29, 2018·World's Poultry Science Journal·Meghali Nighot, Prashant Nighot
Nov 10, 2017·Amino Acids·Artyom Karlovich OsmanyanGleb Yurievich Kosovsky
May 29, 2020·The Journal of Poultry Science·Mikako ShibataYohei Kurose
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Shemil P MacellinePeter H Selle

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