Dietary supplementation with Clostridium butyricum helps to improve the intestinal barrier function of weaned piglets challenged with enterotoxigenic Escherichia coli K88

Journal of Applied Microbiology
H-H LiW-J Wang

Abstract

The objective of this study was twofold: (i) to examine the effect of Clostridium butyricum on intestinal barrier function and (ii) to elucidate the mechanisms involved in enhanced intestinal barrier function. Forty-eight weaned piglets were assigned randomly to either a basal diet or a C. butyricum-supplemented diet. On day 15, all pigs were orally challenged with enterotoxigenic Escherichia coli (ETEC) K88 or saline. Clostridium butyricum decreased serum diamine oxidase activity and d-lactic acid concentration, as well as increased intestinal tight junction proteins (ZO-1, claudin-3 and occludin) expression in ETEC K88-infected pigs. Moreover, C. butyricum decreased IL-1β and IL-18 levels in serum and gut, whereas it increased IL-10 levels. Furthermore, C. butyricum downregulated NLRP3 and caspase-1 expression in ETEC K88-challenged pig gut, but did not affect apoptosis-associated speck-like protein expression. Clostridium butyricum enhanced intestinal barrier function and inhibited apoptosis-associated speck-like protein-independent NLRP3 inflammasome signalling pathway in weaned piglets after ETEC K88 challenge. The novelty of this study lies in the beneficial effects of C. butyricum on intestinal health, likely by improvin...Continue Reading

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Citations

Mar 25, 2020·Journal of Animal Physiology and Animal Nutrition·Jiayun QiaoYupeng Li
Dec 19, 2019·Frontiers in Microbiology·Vanessa C JohannsLothar H Wieler
Jul 15, 2021·Animal Nutrition·Jie PengYanhua Huang
Nov 18, 2021·Journal of Applied Microbiology·Hui HanHongfu Zhang

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