Dietary supplementation with L-arginine limits cell proliferation in the remnant glomerulus

Kidney International
A IngramJ W Scholey

Abstract

One effect of L-arginine is to increase nitric oxide (NO) production by endothelial cells. NO directly reduces endothelin-1 production by endothelial cells and also inhibits platelet-derived growth factor (PDGF) induced cell proliferation. Since subtotal renal ablation is associated with an early phase of cell proliferation in the glomerulus that precedes injury, we examined the effect of dietary supplementation with L-arginine on glomerular cell proliferation and expression of the cytokine endothelin-1 (ET-1). A first group of renal-ablated rats was untreated. A second group of renal-ablated rats received L-arginine (1%) in the drinking water. Two weeks after subtotal ablation renal cortical tissue was snap frozen for immunohistochemical analysis for proliferating cell nuclear antigen (PCNA) expression and ET-1. Protein and total RNA was extracted from sieved glomeruli. mRNA levels were quantitated by co-amplification RT-PCR utilizing specific 5' and 3' primers for rat ET-1 and beta-actin. L-arginine reduced the number of PCNA positive nuclei in remnant glomeruli, and Western blot Analysis of glomerular proteins also showed that L-arginine reduced PCNA expression. Glomerular ET-1 mRNA levels and protein immunostaining declined...Continue Reading

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Citations

Apr 12, 2000·Kidney International·A Benigni
Jul 1, 1997·Kidney International·S AielloG Remuzzi
Apr 9, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·A BenigniG Remuzzi
Mar 2, 2012·Kidney & Blood Pressure Research·Edilia TapiaMartha Franco
Apr 9, 2005·Journal of the American Society of Nephrology : JASN·Joan C KrepinskyAlistair J Ingram
Sep 14, 2000·The Journal of Biological Chemistry·A J IngramJ W Scholey
Jan 5, 2002·The Journal of Biological Chemistry·Joan KrepinskyJames W Scholey
Mar 29, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Y DumontR Larivière
Aug 5, 2000·American Journal of Physiology. Renal Physiology·A J IngramJ W Scholey
Sep 21, 2000·American Journal of Physiology. Renal Physiology·L R JamesJ W Scholey
Oct 22, 2003·Journal of the American Society of Nephrology : JASN·Joan C KrepinskyJames W Scholey
Feb 7, 2001·Journal of the American Society of Nephrology : JASN·Ingrid H C VosJaap A Joles
Dec 26, 2001·Journal of the American Society of Nephrology : JASN·Paola RomagnaniMario Serio

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