Diethylenetriaminopentaacetic acid is unsuitable for long-term preservation of RBCs

Transfusion
T Z LiuA Stern

Abstract

The addition of an appropriate metal chelator, such as diethylenetriaminopentaacetic acid (DTPA) to stored blood has been shown to be effective in a short-term (0-12 days) prevention of lipid peroxidation of stored RBCS: However, its long-term effectiveness has not been carefully evaluated. Blood was preserved in simulated blood bank conditions with or without the addition of DTPA for 4 weeks. Aliquots of stored blood were taken weekly from the storage bag and the deformability profile was determined using a custom-built laser viscodiffractometer. Malondialdehyde (MDA), an index of lipid peroxidation, and the extent of vesiculation of the stored blood were quantified concurrently. It was found that MDA values for DTPA-supplemented blood at the end of a 28-day storage period were significantly elevated compared with the DTPA-free counterpart (23.50 +/- 3.2 vs. 16.10 +/- 2.5 microM; p<0.05). In addition, DTPA-supplemented blood was more susceptible to vesiculation than its DTPA-free counterpart (31.26 +/- 4.1 vs. 10.26 +/- 1.5% of acetyl cholinesterase release, p<0.001). These data are also in accordance with the finding of the deformability profile result, indicating that DTPA-supplemented blood exhibits not only a decrease in d...Continue Reading

References

May 1, 1992·Transfusion·J A KnightH Anstall
Jan 1, 1990·Free Radical Biology & Medicine·H WatanabeN Yamazaki
Dec 28, 1972·The New England Journal of Medicine·C R Valeri, C G Zaroulis
Jan 1, 1984·Methods in Enzymology·K Yagi
Aug 29, 1980·Biochemical and Biophysical Research Communications·D M Lee

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Citations

Jan 21, 2012·Clinical Biochemistry·Mehmet Ramazan ŞekeroğluAydın Him
Mar 9, 2010·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Kuang-Ping PengTsan-Zon Liu

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