Diethylnitrosamine Increases Proliferation in Early Stages of Hepatic Carcinogenesis in Insulin-Treated Type 1 Diabetic Mice

BioMed Research International
A S ArboattiCristina E Carnovale

Abstract

Diethylnitrosamine (DEN) induces hepatocarcinogenesis, increasing mitotic hepatocytes and leading to chronic inflammation. In addition, type 1 diabetes mellitus (T1DM) is also characterized by a proinflammatory state and by requiring insulin exogenous treatment. Given the association of diabetes, insulin treatment, and cell proliferation, our specific goal was to determine whether the liver in the diabetic state presents a greater response to DEN-induced cell cycle alteration, which is essential for the malignant transformation. Male C57BL/6 mice (four-week-old) were divided into 4 groups: C, C + DEN, T1DM, and T1DM + DEN. Mice were euthanized ten weeks after DEN injection. DEN per se produced an increase in liver lipid peroxidation levels. Besides, in T1DM + DEN, we found a greater increase in the proliferation index, in comparison with C + DEN. These results are in agreement with the increased expression observed in cell cycle progression markers: cyclin D1 and E1. In addition, a proapoptotic factor, such as activated caspase-3, evidenced a decrease in T1DM + DEN, while the Vascular Endothelial Growth Factor (VEGF) and the protooncogene p53 showed a higher increase with respect to C + DEN. Overall, the results allow us to hig...Continue Reading

References

Jan 1, 1992·Advances in Immunology·H Kikutani, S Makino
Jan 1, 1990·Methods in Enzymology·H Esterbauer, K H Cheeseman
Aug 1, 1986·Biochemical Pharmacology·C E CarnovaleE A Rodríguez Garay
Sep 1, 1974·The Biochemical Journal·E H KarunanayakeG Mellows
Mar 15, 1984·Experientia·C E Carnovale, E A Rodriguez Garay
Jan 1, 1984·Environmental Mutagenesis·T W Kensler, M A Trush
Jun 1, 1994·Mutation Research·P SchmezerU M Liegibel
Dec 1, 1993·Environmental Health Perspectives·S R EldrigeT L Goldsworthy
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Mar 23, 2000·Journal of Hepatology·N Fausto
Feb 13, 2001·Trends in Biochemical Sciences·J M Adams, S Cory
Dec 26, 2001·Pharmacology & Therapeutics·K C ZimmermannD R Green
Jan 31, 2002·World Journal of Gastroenterology : WJG·Hong-Yu XuLi-Jun Shi
Mar 27, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·María de Luján AlvarezMaría Cristina Carrillo
May 25, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rosa M PascaleFrancesco Feo
Aug 1, 2002·Nature Genetics·Snorri S Thorgeirsson, Joe W Grisham
Oct 24, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Manal M HassanYehuda Z Patt
Feb 26, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tsutomu MasakiShigeki Kuriyama
Dec 5, 2003·Journal of the National Cancer Institute·Kazem ZendehdelWeimin Ye
Sep 16, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·María de Luján AlvarezMaría Cristina Carrillo
Sep 24, 2004·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Eisuke KumeKunio Doi
Mar 25, 2005·Diabetic Medicine : a Journal of the British Diabetic Association·D A Rees, J C Alcolado
Mar 11, 2006·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Hashem B El-SeragFariba Javadi
Aug 25, 2006·Nature Reviews. Cancer·Paraskevi A Farazi, Ronald A DePinho
Jan 24, 2007·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Feb 10, 2007·Chemico-biological Interactions·Kannampalli PradeepSivanesan Karthikeyan
Mar 21, 2007·Biochemical Pharmacology·Ariel D QuirogaMaría Cristina Carrillo
Jun 30, 2007·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·David S H Bell, Eric Allbright
Jul 7, 2007·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sevgi Bozova, Gülsüm Ozlem Elpek
Oct 24, 2007·Chemico-biological Interactions·Venkatabalasubramanian SivaramakrishnanSivasitambaram Niranjali Devaraj
Nov 13, 2007·Gastroenterology Clinics of North America·Prodromos HytiroglouNeil D Theise
Jul 22, 2009·Endocrine-related Cancer·Paolo VigneriRiccardo Vigneri
Aug 8, 2009·International Journal of Experimental Pathology·Femke HeindryckxHans Van Vlierberghe
Aug 29, 2009·Pathology International·Katsuhiro Ogawa
Feb 23, 2010·Seminars in Liver Disease·Nelson Fausto, Jean S Campbell
Jul 6, 2010·Life Sciences·Aparajita Dey, Kavitha Swaminathan
Jun 2, 2011·Toxicologic Pathology·Masahiko KushidaJames E Klaunig

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