Diethylstilbestrol-induced immortalization of human endometrial cells: alterations in p53 and estrogen receptor

Molecular Carcinogenesis
C A RinehartD G Kaufman

Abstract

Carcinogenesis is a process requiring multiple steps. Immortalization is one step in this process and may be rate limiting. To further our understanding of estrogen-induced carcinogenesis, we evaluated diethylstilbestrol (DES)-induced immortalization of human endometrial stromal cells. This was achieved by assessing at the restrictive temperature the colony-forming efficiency of cells that were conditionally immortalized with a temperature-sensitive simian virus 40 large T antigen. Treatment with DES for 1 wk did not increase the immortalization frequency; however, cultures that were treated for 20 wk had a twofold increase in immortalization frequency, and continued treatment for a total of 44 wk produced a threefold increase in immortalization frequency that was dose dependent. DES-treated restrictive temperature variants (RTVs) but not spontaneous RTVs lost the temperature-sensitive phenotype. DES-RTVs also had a shorter doubling time than spontaneous RTVs did. p53 expression was increased in DES-RTVs, and its localization within the cell was altered. Conversely, expression of the estrogen receptor was decreased in DES-immortalized cells. These changes in gene expression often occur in estrogen-related malignancies, and our ...Continue Reading

References

Mar 15, 1979·Nature·D P Lane, L V Crawford
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
Sep 1, 1991·Experimental Cell Research·J W ShayW E Wright
Jun 1, 1991·Contraception·J J Schlesselman
Mar 1, 1991·Archives of Biochemistry and Biophysics·D RoyJ G Liehr
Nov 1, 1990·Cancer Causes & Control : CCC·I PerssonP Sparén
May 16, 1990·Journal of the National Cancer Institute·S A FuquaW L McGuire
Jan 1, 1986·Journal of Steroid Biochemistry·C F HolinkaE Gurpide
Apr 15, 1971·The New England Journal of Medicine·A L HerbstD C Poskanzer
Dec 1, 1983·Molecular and Cellular Biology·N C ReichA J Levine
Sep 15, 1995·International Journal of Cancer. Journal International Du Cancer·L H XuD G Kaufman
Jan 1, 1994·American Journal of Obstetrics and Gynecology·A BerchuckR C Bast
Sep 1, 1993·Carcinogenesis·M A Morse, G D Stoner
Apr 1, 1993·International Journal of Cancer. Journal International Du Cancer·T HondaN Wake
Dec 1, 1961·Experimental Cell Research·L HAYFLICK, P S MOORHEAD

Citations

Apr 1, 1997·Environmental Health Perspectives·T Tsutsui, J C Barrett
Sep 14, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Kitajewski, D Sassoon

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