Difference between orthostatic and march functional proteinuria by application of stress tolerance test and SDS-PAGE

Clinica Chimica Acta; International Journal of Clinical Chemistry
P KornetiB Korneti

Abstract

We introduced minimal necessary criteria and methods for noninvasive laboratory diagnosis and follow-up of functional proteinuria in youths: stress tolerance test, determination of total urinary proteins (TP) and their separation and identification with gradient SDS-PAGE. Renal functional adaptability in conditions of complete rest, in routine daily activity and after several hours of active physical effort has been evaluated by the tolerance test. Excretory urinary proteins, as the most appropriate markers, were analyzed with noninvasive methods. Excretory TP demonstrated the quantity and dynamics of the proteins excreted during the tolerance test. Separation with gradient (4-22.5%) SDS-PAGE provided differentiation of functional proteinuria from the other orthostatisms, through the protein fractions present and the constant finding of apolipoprotein AI. The investigation comprised 19 youths with orthostatism and 20 healthy subjects without orthostatism, all between the age of 10 and 18 years. The subjects without orthostatism excreted proteins in normal limits during the stress tolerance test. SDS-PAGE of the urinary samples, obtained during the tolerance test, in five subjects significantly differed from that in the other 14...Continue Reading

References

Jan 1, 1977·International Urology and Nephrology·J Buchanec, K Javorka
Jul 1, 1990·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·N ShintakuY Kuroda
Jul 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·T BrocklebankK Richmond
Sep 13, 1991·Journal of Chromatography·A Bianchi-BosisioP G Righetti
Mar 1, 1990·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M YoshimotoM Sudo
May 1, 1988·Metabolism: Clinical and Experimental·D Reichl, R Beetham
Oct 1, 1982·Annals of Internal Medicine·P D SpringbergR R Robinson
Jan 1, 1981·Scandinavian Journal of Urology and Nephrology·M von BonsdorffA Pasternack
Dec 1, 1994·Mayo Clinic Proceedings·T S Larson
Jan 1, 1997·The Journal of Laboratory and Clinical Medicine·G RamjeeM Adhikari
Apr 1, 1997·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·S J LeeE C Chung
Sep 1, 1960·Clinica Chimica Acta; International Journal of Clinical Chemistry·O MEULEMANS

Citations

Aug 17, 2001·Clinical Chemistry and Laboratory Medicine : CCLM·V TasicB Korneti

Related Concepts

Adaptation, Physiological
Teens
SDS-PAGE
Proteinuria
Metabolic Stress Response
Exercise, Isometric

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.