PMID: 12780699Jun 5, 2003Paper

Difference in antigenic determinant profiles between human and rat myeloperoxidase

Clinical and Experimental Immunology
Y C PatryV L Esnault

Abstract

We tested whether rat and human MPO have similar antigenic determinants using 36 human MPO-ANCA positive sera, one mouse anti-rat MPO and four mouse anti-human MPO monoclonal reagents. Purified rat and human MPO were used in ELISA, with or without crossinhibition by preincubation with human MPO or irrelevant antigen in the liquid phase. Only one human MPO ANCA positive serum exhibited significant binding in rat MPO ELISA. This binding was poorly inhibited by preincubation with human MPO in the liquid phase, but was conserved after adsorption of non specific anti-rat activity in a chromatography column. Three mouse anti-human MPO IgG monoclonal antibodies did not recognize rat MPO. Only one mouse anti-human MPO IgA monoclonal antibody bound to rat MPO. This binding was poorly inhibited by preincubation with human MPO (35% at 2 micro g/ml). Conversely, the mouse anti-rat MPO monoclonal did not bind human MPO. We have concluded that: (1) Most human MPO-ANCA recognize antigenic determinants on human MPO which are absent on rat MPO. Therefore, human auto-antibodies bind to epitopes which recently appeared after species evolution; (2) Inversely, the mouse anti-rat MPO monoclonal do not bind human MPO. Therefore, rat MPO epitopes have...Continue Reading

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Citations

Jul 2, 2009·American Journal of Veterinary Research·Barrak M PresslerKathleen I Anderson
Oct 25, 2016·The Journal of Nutritional Biochemistry·Olga KrupkovaKarin Wuertz-Kozak
Mar 25, 2006·Kidney International·U ErdbrüggerP H Nachman
Aug 24, 2004·Clinical and Experimental Immunology·Y M van der GeldC G M Kallenberg

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