Difference in distribution of membrane proteins between low- and high-density secretory granules in parotid acinar cells

Biochemical and Biophysical Research Communications
Junko Fujita-YoshigakiHiroshi Sugiya

Abstract

Secretory granules (SGs) are considered to be generated as immature granules and to mature by condensation of their contents. In this study, SGs of parotid gland were separated into low-, medium-, and high-density granule fractions by Percoll-density gradient centrifugation, since it was proposed that the density corresponds to the degree of maturation. The observation with electron microscopy showed that granules in the three fractions were very similar. The average diameter of high-density granules was a little but significantly larger than that of low-density granules. Although the three fractions contained amylase, suggesting that they are all SGs, distribution of membrane proteins was markedly different. Syntaxin6 and VAMP4 were localized in the low-density granule fraction, while VAMP2 was concentrated in the high-density granule fraction. Immunoprecipitation with anti-syntaxin6 antibody caused coprecipitation of VAMP2 from the medium-density granule fraction without solubilization, but not from Triton X-100-solubilized fraction, while VAMP4 was coprecipitated from both fractions. Therefore, VAMP2 is present on the same granules, but is separated from syntaxin6 and VAMP4, which are expected to be removed from immature gra...Continue Reading

References

Dec 1, 1991·The Journal of Cell Biology·S A ToozeW B Huttner
Jul 1, 1997·Molecular Biology of the Cell·J B BockR H Scheller
Aug 29, 1998·Archives of Biochemistry and Biophysics·Y DohkeS Furuyama
Dec 29, 1998·The Journal of Cell Biology·S UrbéS A Tooze
Jan 19, 1999·Annual Review of Cell and Developmental Biology·D A Brown, E London
Oct 3, 1999·The Journal of Biological Chemistry·A SimonsenH Stenmark
Apr 18, 2001·Trends in Cell Biology·S A ToozeW B Huttner
Jun 16, 2001·Molecular Biology of the Cell·F WendlerS A Tooze
Sep 14, 2001·Traffic·F Wendler, S Tooze
Feb 13, 2002·The Journal of Cell Biology·Frédéric MallardLudger Johannes
Aug 23, 2002·Molecular and Cellular Biology·Dietmar RiedelReinhard Jahn
Nov 1, 1992·Trends in Cell Biology·P Arvan, D Castle
Jan 27, 2004·Molecular Biology of the Cell·Regina KuliawatPeter Arvan

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Citations

Oct 27, 2010·Expert Review of Proteomics·María Gómez-LázaroMichael Schrader
Apr 27, 2007·Biochemical and Biophysical Research Communications·Osamu KatsumataHiroshi Sugiya
Aug 2, 2011·European Journal of Immunology·Konrad KrzewskiJack L Strominger
Jan 1, 2009·The Journal of Medical Investigation : JMI·Osamu Katsumata-KatoHiroshi Sugiya
Sep 16, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hiroshi GomiAzuma Tsukise
Sep 14, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Junko Fujita-YoshigakiOsamu Katsumata-Kato

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